Repair of Long Nerve Defects with a New Decellularized Nerve Graft in Rats and in Sheep

Estefanía Contreras, Sara Traserra, Sara Bolívar, Joaquim Forés, Eduard Jose-Cunilleras, Felix García, Ignacio Delgado-Martínez, Sandra Holmgren, Raimund Strehl, Esther Udina, Xavier Navarro

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Decellularized nerve allografts (DC) are an alternative to autografts (AG) for repairing severe peripheral nerve injuries. We have assessed a new DC provided by VERIGRAFT. The decellularization procedure completely removed cellularity while preserving the extracellular matrix. We first assessed the DC in a 15 mm gap in the sciatic nerve of rats, showing slightly delayed but effective regeneration. Then, we assayed the DC in a 70 mm gap in the peroneal nerve of sheep compared with AG. Evaluation of nerve regeneration and functional recovery was performed by clinical, electrophysiology and ultrasound tests. No significant differences were found in functional recovery between groups of sheep. Histology showed a preserved fascicular structure in the AG while in the DC grafts regenerated axons were grouped in small units. In conclusion, the DC was permissive for axonal regeneration and allowed to repair a 70 mm long gap in the sheep nerve.
Original languageUndefined/Unknown
Article number4074
Number of pages19
JournalCells
Volume11
Issue number24
DOIs
Publication statusPublished - Dec 2022

Cite this