Renal Mechanisms Involved in Stress-induced Antinatriuresis and Antidiuresis in Rats

M. L. Bertuzzi, N. Bensi, N. Mayer, A. Niebylski, A. Armario, H. F. Gauna

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13 Citations (Scopus)


The present study was conducted to investigate if changes in sodium and water excretion in stressed animals were due to modifications in the glomerular filtration rate (GFR) and to determine the participation of angiotensin II (Ang II) and α and β-adrenoceptors on sodium and water renal excretion in rats subjected to immobilization stress (IMO). Male Wistar rats (250-300 g) were randomly separated into five different groups and vehicle (0.9% NaCl) via intraperitoneal (i.p.) or propanolol (3 mg/kg i.p.) or captopril (6 mg/kg i.p.) or yohimbine (3 mg/kg i.p.) or prazosin (1 mg/kg i.p.) were injected respectively. During experimental measurements, the animals were kept in metabolic cages for 6 h and sodium, potassium and water renal excretion and saline (1.5% NaCl) and water intake were determinated at day 1 (drug effect) and day 7 (drug + IMO effects). GFR was measured by creatinine clearance in control and IMO rats. A stress-induced antinatriuresis and antidiuresis was reversed by α1 and α2-adrenoceptor antagonists, while captopril inhibited only the antidiuresis and propranolol had no effect on either parameter. No differences were observed in creatinine clearance in the studied groups. Since yohimbine blocks α 2-adrenoceptors and prazosin blocks α1- adrenoceptors and α2B-adrenoceptors, the stress-induced renal sodium reabsorption mainly could be attributed to α2B- adrenoceptors. The present results indicate that β-adrenoceptors do not participate in this response and, Ang II only reverses the antidiuresis and shows a slight participation in antinatriuresis. The increment in sodium and water reabsorption caused by IMO occurred without changes in the glomerular filtration rate.
Original languageEnglish
Pages (from-to)259-264
JournalArchives of Physiology and Biochemistry
Issue number3
Publication statusPublished - 1 Jul 2003


  • α-adrenoceptor antagonist
  • Captopril
  • Immobilization stress
  • Rats
  • Sodium excretion


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