TY - JOUR
T1 - Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments
AU - Rodríguez-López, Pedro
AU - Carballo-Justo, Alba
AU - Draper, Lorraine A.
AU - Cabo, Marta L.
PY - 2017/1/2
Y1 - 2017/1/2
N2 - © 2016 Informa UK Limited, trading as Taylor & Francis Group. The effects of pronase (PRN), cellulase (CEL) or DNaseI alone or combined with benzalkonium chloride (BAC) against Listeria monocytogenes-carrying biofilms were assayed. The best removal activity against L. monocytogenes–Escherichia coli biofilms was obtained using DNaseI followed by PRN and CEL. Subsequently, a modified logistic model was used to quantify the combined effects of PRN or DNaseI with BAC. A better BAC performance after PRN compared to DNaseI eradicating L. monocytogenes was observed. In E. coli the effects were the opposite. Finally, effects of DNaseI and DNaseI–BAC treatments were compared against two different L. monocytogenes-carrying biofilms. DNaseI–BAC was more effective against L. monocytogenes when co-cultured with E. coli. Nonetheless, comparing the removal effects after BAC addition, these were higher in mixed-biofilms with Pseudomonas fluorescens. However, a high number of released viable cells was observed after combined treatments. These results open new perspectives of enzymes as an anti-biofilm strategy for environmental pathogen control.
AB - © 2016 Informa UK Limited, trading as Taylor & Francis Group. The effects of pronase (PRN), cellulase (CEL) or DNaseI alone or combined with benzalkonium chloride (BAC) against Listeria monocytogenes-carrying biofilms were assayed. The best removal activity against L. monocytogenes–Escherichia coli biofilms was obtained using DNaseI followed by PRN and CEL. Subsequently, a modified logistic model was used to quantify the combined effects of PRN or DNaseI with BAC. A better BAC performance after PRN compared to DNaseI eradicating L. monocytogenes was observed. In E. coli the effects were the opposite. Finally, effects of DNaseI and DNaseI–BAC treatments were compared against two different L. monocytogenes-carrying biofilms. DNaseI–BAC was more effective against L. monocytogenes when co-cultured with E. coli. Nonetheless, comparing the removal effects after BAC addition, these were higher in mixed-biofilms with Pseudomonas fluorescens. However, a high number of released viable cells was observed after combined treatments. These results open new perspectives of enzymes as an anti-biofilm strategy for environmental pathogen control.
KW - Benzalkonium chloride
KW - biofilm
KW - disinfection
KW - enzymes
KW - fluorescence microscopy
KW - Listeria monocytogenes
U2 - 10.1080/08927014.2016.1261847
DO - 10.1080/08927014.2016.1261847
M3 - Article
VL - 33
SP - 45
EP - 58
JO - Biofouling
JF - Biofouling
SN - 0892-7014
IS - 1
ER -