Removal of Listeria monocytogenes dual-species biofilms using combined enzyme-benzalkonium chloride treatments

Pedro Rodríguez-López, Alba Carballo-Justo, Lorraine A. Draper, Marta L. Cabo

Research output: Contribution to journalArticleResearchpeer-review

40 Citations (Scopus)


© 2016 Informa UK Limited, trading as Taylor & Francis Group. The effects of pronase (PRN), cellulase (CEL) or DNaseI alone or combined with benzalkonium chloride (BAC) against Listeria monocytogenes-carrying biofilms were assayed. The best removal activity against L. monocytogenes–Escherichia coli biofilms was obtained using DNaseI followed by PRN and CEL. Subsequently, a modified logistic model was used to quantify the combined effects of PRN or DNaseI with BAC. A better BAC performance after PRN compared to DNaseI eradicating L. monocytogenes was observed. In E. coli the effects were the opposite. Finally, effects of DNaseI and DNaseI–BAC treatments were compared against two different L. monocytogenes-carrying biofilms. DNaseI–BAC was more effective against L. monocytogenes when co-cultured with E. coli. Nonetheless, comparing the removal effects after BAC addition, these were higher in mixed-biofilms with Pseudomonas fluorescens. However, a high number of released viable cells was observed after combined treatments. These results open new perspectives of enzymes as an anti-biofilm strategy for environmental pathogen control.
Original languageEnglish
Pages (from-to)45-58
Issue number1
Publication statusPublished - 2 Jan 2017


  • Benzalkonium chloride
  • biofilm
  • disinfection
  • enzymes
  • fluorescence microscopy
  • Listeria monocytogenes


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