Rehosting of bacterial chaperones for high-quality protein production

Mónica Martínez-Alonso, Verónica Toledo-Rubio, Rob Noad, Ugutz Unzueta, Neus Ferrer-Miralles, Polly Roy, Antonio Villaverde

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

Coproduction of DnaK/DnaJ in Escherichia coli enhances solubility but promotes proteolytic degradation of their substrates, minimizing the yield of unstable polypeptides. Higher eukaryotes have orthologs of DnaK/DnaJ but lack the linked bacterial proteolytic system. By coexpression of DnaK and DnaJ in insect cells with inherently misfolding-prone recombinant proteins, we demonstrate simultaneous improvement of soluble protein yield and quality and proteolytic stability. Thus, undesired side effects of bacterial folding modulators can be avoided by appropriate rehosting in heterologous cell expression systems. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)7850-7854
JournalApplied and Environmental Microbiology
Volume75
Issue number24
DOIs
Publication statusPublished - 1 Dec 2009

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