TY - JOUR
T1 - Regulatory T cells and other lymphocyte subpopulations in patients with melanoma developing interferon-induced thyroiditis during high-dose interferon-α2b treatment
AU - Soldevila, Berta
AU - Alonso, Núria
AU - Martínez-Arconada, Maria J.
AU - Granada, Maria L.
AU - Boada, Aram
AU - Vallejos, Virginia
AU - Fraile, Manuel
AU - Fernández-Sanmartín, Marco A.
AU - Pujol-Borrell, Ricardo
AU - Puig-Domingo, Manel
AU - Sanmartí, Anna
AU - Martínez-Cáceres, Eva M.
PY - 2013/4/1
Y1 - 2013/4/1
N2 - Context One of the side effects of interferon-alpha therapy is interferon-induced thyroiditis (IIT). The role of lymphocyte subpopulations in IIT melanoma patients remains to be defined. Objective Our objective was to assess different peripheral blood lymphocyte subpopulations, mainly regulatory T cells (Tregs), in melanoma patients who developed IIT. Design, patients and methods From 30 melanoma patients receiving high-dose interferon (HDI)-alpha 2b (IFN-α2b) treatment, those who developed IIT (IIT patients) were selected and compared with patients who did not develop IIT (Co-MM) and healthy controls (Co-H). Peripheral blood mononuclear cells were obtained before treatment (BT), mid-treatment (MT), end of treatment (ET), 24 weeks post-treatment and at appearance of IIT (TT). Results Nine patients developed IIT (30%): four Hashimoto's thyroiditis and five destructive thyroiditis. An increase in Tregs was observed in both melanoma groups during HDI treatment. A decrease in CD3+, NKT lymphocyte subpopulations and Bcl2 expression on B cells was also observed in both groups. However, no changes were observed in the percentage of CD4+, CD8+, CD3+γδ +, CD19+, transitional B cells (CD24 highCD38highCD19+CD27-), natural killer (NK), invariant NKT (iNKT) lymphocytes and Th1/Th2 balance when BT was compared with ET. At TT, IIT patients had a higher Tregs percentage than Co-MM (P = 0.012) and Co-H (P = 0.004), a higher iNKT percentage than Co-MM (P = 0.011), a higher transitional B cells percentage than Co-H (P = 0.015), a lower CD3+ percentage than Co-H (P = 0.001) and a lower Bcl2 expression on B cells than Co-H (P < 0.001). Conclusions: Our results point to the immunomodulatory effects of IFN-α on different lymphocyte subpopulations and a possible role of Tregs in melanoma patients who developed IIT. © 2012 Blackwell Publishing Ltd.
AB - Context One of the side effects of interferon-alpha therapy is interferon-induced thyroiditis (IIT). The role of lymphocyte subpopulations in IIT melanoma patients remains to be defined. Objective Our objective was to assess different peripheral blood lymphocyte subpopulations, mainly regulatory T cells (Tregs), in melanoma patients who developed IIT. Design, patients and methods From 30 melanoma patients receiving high-dose interferon (HDI)-alpha 2b (IFN-α2b) treatment, those who developed IIT (IIT patients) were selected and compared with patients who did not develop IIT (Co-MM) and healthy controls (Co-H). Peripheral blood mononuclear cells were obtained before treatment (BT), mid-treatment (MT), end of treatment (ET), 24 weeks post-treatment and at appearance of IIT (TT). Results Nine patients developed IIT (30%): four Hashimoto's thyroiditis and five destructive thyroiditis. An increase in Tregs was observed in both melanoma groups during HDI treatment. A decrease in CD3+, NKT lymphocyte subpopulations and Bcl2 expression on B cells was also observed in both groups. However, no changes were observed in the percentage of CD4+, CD8+, CD3+γδ +, CD19+, transitional B cells (CD24 highCD38highCD19+CD27-), natural killer (NK), invariant NKT (iNKT) lymphocytes and Th1/Th2 balance when BT was compared with ET. At TT, IIT patients had a higher Tregs percentage than Co-MM (P = 0.012) and Co-H (P = 0.004), a higher iNKT percentage than Co-MM (P = 0.011), a higher transitional B cells percentage than Co-H (P = 0.015), a lower CD3+ percentage than Co-H (P = 0.001) and a lower Bcl2 expression on B cells than Co-H (P < 0.001). Conclusions: Our results point to the immunomodulatory effects of IFN-α on different lymphocyte subpopulations and a possible role of Tregs in melanoma patients who developed IIT. © 2012 Blackwell Publishing Ltd.
U2 - 10.1111/cen.12036
DO - 10.1111/cen.12036
M3 - Article
SN - 0300-0664
VL - 78
SP - 621
EP - 628
JO - Clinical Endocrinology
JF - Clinical Endocrinology
ER -