Regulation of Trk-dependent potassium transport by the calcineurin pathway involves the Hal5 kinase

Carlos Casado, Lynne Yenush, Carmen Melero, María del Carmen Ruiz, Raquel Serrano, Jorge Pérez-Valle, Joaquín Ariño, José Ramos

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19 Citations (Scopus)

Abstract

The phosphatase calcineurin and the kinases Hal4/Hal5 regulate high-affinity potassium uptake in Saccharomyces cerevisiae through the Trk1 transporter. We demonstrate that calcineurin is necessary for high-affinity potassium uptake even in the absence of Na+ stress. HAL5 expression is induced in response to stress in a calcineurin-dependent manner through a newly identified functional CDRE (nt -195/-189). Lack of calcineurin decreases Hal5 protein levels, although with little effect on Trk1 amounts. However, the growth defect of cnb1 cells at K+-limiting conditions can be rescued in part by overexpression of HAL5, and this mutation further aggravates the potassium requirements of a hal4 strain. This suggests that the control exerted by calcineurin on Hal5 expression may be biologically relevant for Trk1 regulation. © 2010 Federation of European Biochemical Societies.
Original languageEnglish
Pages (from-to)2415-2420
JournalFEBS Letters
Volume584
DOIs
Publication statusPublished - 1 Jun 2010

Keywords

  • Gene expression
  • High-affinity potassium transport
  • Saccharomyces cerevisiae
  • Trk1

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