Reconstructing 3D light microscopic images using the EM algorithm

Jordi Vitrià, Jorge Llacer

    Research output: Contribution to journalArticleResearchpeer-review

    6 Citations (Scopus)


    An iterative method to recover perfectly focused images from a set of light microscopic images is proposed. The method is based on the EM algorithm, and it assumes a prior knowledge about the Point Spread Function of the optical system, as well as about the optical parameter settings of the acquisition system. The method is applied to the visualization of integrated circuit samples through an optical microscope and to the recovery of their depth information.
    Original languageEnglish
    Pages (from-to)1491-1498
    JournalPattern Recognition Letters
    Issue number14
    Publication statusPublished - 30 Dec 1996


    • Depth from focus
    • EM algorithm
    • Image restoration
    • Optical microscopy


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