TY - JOUR
T1 - Recombinant PrPScshares structural features with brain-derived PrPSc: Insights from limited proteolysis
AU - Sevillano, Alejandro M.
AU - Fernández-Borges, Natalia
AU - Younas, Neelam
AU - Wang, Fei
AU - R. Elezgarai, Saioa
AU - Bravo, Susana
AU - Vázquez-Fernández, Ester
AU - Rosa, Isaac
AU - Eraña, Hasier
AU - Gil, David
AU - Veiga, Sonia
AU - Vidal, Enric
AU - Erickson-Beltran, Melissa L.
AU - Guitián, Esteban
AU - Silva, Christopher J.
AU - Nonno, Romolo
AU - Ma, Jiyan
AU - Castilla, Joaquín
AU - R. Requena, Jesús
PY - 2018/1/1
Y1 - 2018/1/1
N2 - © 2018 Public Library of Science. All Rights Reserved. Very solid evidence suggests that the core of full length PrPScis a 4-rung β-solenoid, and that individual PrPScsubunits stack to form amyloid fibers. We recently used limited proteolysis to map the β-strands and connecting loops that make up the PrPScsolenoid. Using high resolution SDS-PAGE followed by epitope analysis, and mass spectrometry, we identified positions ~116/118, 133–134, 141, 152–153, 162, 169 and 179 (murine numbering) as Proteinase K (PK) cleavage sites in PrPSc. Such sites likely define loops and/or borders of β-strands, helping us to predict the threading of the β-solenoid. We have now extended this approach to recombinant PrPSc(recPrPSc). The term recPrPScrefers to bona fide recombinant prions prepared by PMCA, exhibiting infectivity with attack rates of ~100%. Limited proteolysis of mouse and bank vole recPrPScspecies yielded N-terminally truncated PK-resistant fragments similar to those seen in brain-derived PrPSc, albeit with varying relative yields. Along with these fragments, doubly N- and C-terminally truncated fragments, in particular ~89/97-152, were detected in some recPrPScpreparations; similar fragments are characteristic of atypical strains of brain-derived PrPSc. Our results suggest a shared architecture of recPrPScand brain PrPScprions. The observed differences, in particular the distinct yields of specific PK-resistant fragments, are likely due to differences in threading which result in the specific biochemical characteristics of recPrPSc. Furthermore, recombinant PrPScoffers exciting opportunities for structural studies unachievable with brain-derived PrPSc.
AB - © 2018 Public Library of Science. All Rights Reserved. Very solid evidence suggests that the core of full length PrPScis a 4-rung β-solenoid, and that individual PrPScsubunits stack to form amyloid fibers. We recently used limited proteolysis to map the β-strands and connecting loops that make up the PrPScsolenoid. Using high resolution SDS-PAGE followed by epitope analysis, and mass spectrometry, we identified positions ~116/118, 133–134, 141, 152–153, 162, 169 and 179 (murine numbering) as Proteinase K (PK) cleavage sites in PrPSc. Such sites likely define loops and/or borders of β-strands, helping us to predict the threading of the β-solenoid. We have now extended this approach to recombinant PrPSc(recPrPSc). The term recPrPScrefers to bona fide recombinant prions prepared by PMCA, exhibiting infectivity with attack rates of ~100%. Limited proteolysis of mouse and bank vole recPrPScspecies yielded N-terminally truncated PK-resistant fragments similar to those seen in brain-derived PrPSc, albeit with varying relative yields. Along with these fragments, doubly N- and C-terminally truncated fragments, in particular ~89/97-152, were detected in some recPrPScpreparations; similar fragments are characteristic of atypical strains of brain-derived PrPSc. Our results suggest a shared architecture of recPrPScand brain PrPScprions. The observed differences, in particular the distinct yields of specific PK-resistant fragments, are likely due to differences in threading which result in the specific biochemical characteristics of recPrPSc. Furthermore, recombinant PrPScoffers exciting opportunities for structural studies unachievable with brain-derived PrPSc.
U2 - 10.1371/journal.ppat.1006797
DO - 10.1371/journal.ppat.1006797
M3 - Article
C2 - 29385212
VL - 14
JO - PLoS Pathogens
JF - PLoS Pathogens
SN - 1553-7366
IS - 1
M1 - e1006797
ER -