This study was undertaken to determine if administration of recombinant bovine interleukin-12 (rBoIL-12) could stimulate a cellular immune response that protected calves from an oral challenge inoculation with Cryptosporidium parvum oocysts. In a first experiment, rBoIL-12 intraperitoneally administered as a single dose 1 day before challenge inoculation, did not alter the course of infection. The percentage of immune competent cells and levels of cytokine gene expression in the ileo-cecal mucosa and in the draining lymph nodes of treated calves were similar to those of untreated control calves. However, when rBoIL-12 was subcutaneously administered daily from 2 days before infection to 2 days after infection, a consistent increase of T lymphocytes and an higher expression of interferon-γ (IFN-γ) was detected. Again, treatment did not alter the course of infection. Similar results were obtained when rBoIL-12 was administered daily for 4 days beginning 2 days after oral inoculation. These data indicate that although rBoIL-12 stimulated a strong immune response in the gut of neonatal calves, the response was not able to provide protection from challenge inoculation with C. parvum oocysts.
|Journal||Veterinary Parasitology: Regional Studies and Reports|
|Publication status||Published - 18 Feb 2006|
- Cellular immune response
- Cryptosporidium parvum
Pasquali, P., Fayer, R., Zarlenga, D., Canals, A., De Marez, T., Gomez Munoz, M. T., Almeria, S., & Gasbarre, L. C. (2006). Recombinant bovine interleukin-12 stimulates a gut immune response but does not provide resistance to Cryptosporidium parvum infection in neonatal calves. Veterinary Parasitology: Regional Studies and Reports, 135, 259-268. https://doi.org/10.1016/j.vetpar.2005.05.062