Recombinant bovine interleukin-12 stimulates a gut immune response but does not provide resistance to Cryptosporidium parvum infection in neonatal calves

Paolo Pasquali, Ronald Fayer, Dante Zarlenga, Ana Canals, Tine De Marez, Maria Teresa Gomez Munoz, Sonia Almeria, Louis C. Gasbarre

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

This study was undertaken to determine if administration of recombinant bovine interleukin-12 (rBoIL-12) could stimulate a cellular immune response that protected calves from an oral challenge inoculation with Cryptosporidium parvum oocysts. In a first experiment, rBoIL-12 intraperitoneally administered as a single dose 1 day before challenge inoculation, did not alter the course of infection. The percentage of immune competent cells and levels of cytokine gene expression in the ileo-cecal mucosa and in the draining lymph nodes of treated calves were similar to those of untreated control calves. However, when rBoIL-12 was subcutaneously administered daily from 2 days before infection to 2 days after infection, a consistent increase of T lymphocytes and an higher expression of interferon-γ (IFN-γ) was detected. Again, treatment did not alter the course of infection. Similar results were obtained when rBoIL-12 was administered daily for 4 days beginning 2 days after oral inoculation. These data indicate that although rBoIL-12 stimulated a strong immune response in the gut of neonatal calves, the response was not able to provide protection from challenge inoculation with C. parvum oocysts.
Original languageEnglish
Pages (from-to)259-268
JournalVeterinary Parasitology: Regional Studies and Reports
Volume135
DOIs
Publication statusPublished - 18 Feb 2006

Keywords

  • Cattle
  • Cellular immune response
  • Cryptosporidiosis
  • Cryptosporidium parvum
  • Cytokine
  • Interleukin-12

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