Abstract
Efficient development of enzymatic synthesis in two-phase systems is closely related with appropriate selection of the reaction medium (especially the solvent and phase ratio). A selection procedure based on the calculation of the theoretically allowable conversion and product concentration is presented and applied to a peptide synthesis using papain. For the synthesis of the dipeptide Boc-Gly-Phe-OMe, the operating conditions have been determined, and the two-phase system to be used has been successfully selected (with trichloroethylene being the best solvent). © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Original language | English |
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Pages (from-to) | 49-60 |
Journal | Biocatalysis and Biotransformation |
Volume | 7 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1 Jan 1992 |
Keywords
- Enzymatic peptide synthesis
- Extractive biocatalysis
- Papain
- Reaction medium engineering
- Two-phase system