Reaction medium selection for an enzymatic peptide synthesis in an aqueous-organic two-phase system

Montserrat Sarrà, Glòria Caminal, Glória González, Josep López-Santín

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7 Citations (Scopus)

Abstract

Efficient development of enzymatic synthesis in two-phase systems is closely related with appropriate selection of the reaction medium (especially the solvent and phase ratio). A selection procedure based on the calculation of the theoretically allowable conversion and product concentration is presented and applied to a peptide synthesis using papain. For the synthesis of the dipeptide Boc-Gly-Phe-OMe, the operating conditions have been determined, and the two-phase system to be used has been successfully selected (with trichloroethylene being the best solvent). © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Original languageEnglish
Pages (from-to)49-60
JournalBiocatalysis and Biotransformation
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Jan 1992

Keywords

  • Enzymatic peptide synthesis
  • Extractive biocatalysis
  • Papain
  • Reaction medium engineering
  • Two-phase system

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