Reaction medium selection for an enzymatic peptide synthesis in an aqueous-organic two-phase system

Montserrat Sarrà; Glòria Caminal; Glória González; Josep López-Santín

doi:10.3109/10242429209003661

Reaction medium selection for an enzymatic peptide synthesis in an aqueous-organic two-phase system

Montserrat Sarrà, Glòria Caminal, Glória González, Josep López-Santín

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

7 Citations (Scopus)

Abstract

Efficient development of enzymatic synthesis in two-phase systems is closely related with appropriate selection of the reaction medium (especially the solvent and phase ratio). A selection procedure based on the calculation of the theoretically allowable conversion and product concentration is presented and applied to a peptide synthesis using papain. For the synthesis of the dipeptide Boc-Gly-Phe-OMe, the operating conditions have been determined, and the two-phase system to be used has been successfully selected (with trichloroethylene being the best solvent). © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Original language	English
Pages (from-to)	49-60
Journal	Biocatalysis and Biotransformation
Volume	7
Issue number	1
DOIs	https://doi.org/10.3109/10242429209003661
Publication status	Published - 1 Jan 1992

Keywords

Enzymatic peptide synthesis
Extractive biocatalysis
Papain
Reaction medium engineering
Two-phase system

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title = "Reaction medium selection for an enzymatic peptide synthesis in an aqueous-organic two-phase system",

abstract = "Efficient development of enzymatic synthesis in two-phase systems is closely related with appropriate selection of the reaction medium (especially the solvent and phase ratio). A selection procedure based on the calculation of the theoretically allowable conversion and product concentration is presented and applied to a peptide synthesis using papain. For the synthesis of the dipeptide Boc-Gly-Phe-OMe, the operating conditions have been determined, and the two-phase system to be used has been successfully selected (with trichloroethylene being the best solvent). {\textcopyright} 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.",

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AU - Sarrà, Montserrat

AU - Caminal, Glòria

AU - González, Glória

AU - López-Santín, Josep

PY - 1992/1/1

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N2 - Efficient development of enzymatic synthesis in two-phase systems is closely related with appropriate selection of the reaction medium (especially the solvent and phase ratio). A selection procedure based on the calculation of the theoretically allowable conversion and product concentration is presented and applied to a peptide synthesis using papain. For the synthesis of the dipeptide Boc-Gly-Phe-OMe, the operating conditions have been determined, and the two-phase system to be used has been successfully selected (with trichloroethylene being the best solvent). © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

AB - Efficient development of enzymatic synthesis in two-phase systems is closely related with appropriate selection of the reaction medium (especially the solvent and phase ratio). A selection procedure based on the calculation of the theoretically allowable conversion and product concentration is presented and applied to a peptide synthesis using papain. For the synthesis of the dipeptide Boc-Gly-Phe-OMe, the operating conditions have been determined, and the two-phase system to be used has been successfully selected (with trichloroethylene being the best solvent). © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

KW - Enzymatic peptide synthesis

KW - Extractive biocatalysis

KW - Papain

KW - Reaction medium engineering

KW - Two-phase system

U2 - 10.3109/10242429209003661

DO - 10.3109/10242429209003661

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EP - 60

JO - Biocatalysis and Biotransformation

JF - Biocatalysis and Biotransformation

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