Efficient development of enzymatic synthesis in two-phase systems is closely related with appropriate selection of the reaction medium (especially the solvent and phase ratio). A selection procedure based on the calculation of the theoretically allowable conversion and product concentration is presented and applied to a peptide synthesis using papain. For the synthesis of the dipeptide Boc-Gly-Phe-OMe, the operating conditions have been determined, and the two-phase system to be used has been successfully selected (with trichloroethylene being the best solvent). © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
|Journal||Biocatalysis and Biotransformation|
|Publication status||Published - 1 Jan 1992|
- Enzymatic peptide synthesis
- Extractive biocatalysis
- Reaction medium engineering
- Two-phase system