Reaction engineering for consecutive enzymatic reactions in peptide synthesis: Application to the synthesis of a pentapeptide

Salvador Ruiz, Josep A. Feliu, Glòria Caminal, Gregorio Álvaro, Josep López-Santín

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

A single-pot enzymatic synthesis of Z-CCK5 (4-8) is presented in this work, employing Z-Gly-Trp-OBzl as acyl donor, under kinetic control. The first goal of the work is the development of a synthetic strategy allowing the use of the same medium for two reactions catalyzed by immobilized α-chymotrypsin, discriminating between simultaneous and consecutive addition systems. The second goal is the maximization of the pentapeptide yield as a function of the molar excess of both nucleophiles employed. A maximum yield of 36% was obtained, and the addition strategy as well as the optimal initial concentrations of substrates have been determined.
Original languageEnglish
Pages (from-to)783-787
JournalBiotechnology Progress
Volume13
DOIs
Publication statusPublished - 1 Nov 1997

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