Rational strategy for the production of new crude lipases from Candida rugosa

Pablo Domínguez De María, José M. Sánchez-Montero, Andrés R. Alcántara, Francisco Valero, José V. Sinisterra

    Research output: Contribution to journalArticleResearchpeer-review

    15 Citations (Scopus)

    Abstract

    Candida rugosa was cultured using different inducers (oleic acid, olive oil, sunflower oil, n-dodecanol and glycerol) as the only carbon source in batch conditions, as well as in several fed-batch fermentations (oleic acid as inducer) at variable feed rate conditions. The N-terminal analysis of each crude lipase revealed that, while the isoenzymes Lip2 and Lip3 are always secreted (at different proportions depending on the inducer), Lip1 was produced only using n-dodecanol (batch conditions) or oleic acid (fed-batch at high feed rate). The nature of the inducer controls the isoenzyme percentage; when this is fixed, as well as the feed rate in fed-batch fermentation, the isoenzymatic profile remained unaltered and the samples differed only in the activity of the lipases, as determined by heptyl oleate synthesis. © Springer 2005.
    Original languageEnglish
    Pages (from-to)499-503
    JournalBiotechnology Letters
    Volume27
    DOIs
    Publication statusPublished - 1 Apr 2005

    Keywords

    • Biotransformations
    • Candida rugosa
    • Isoenzymes
    • Lipases

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