TY - JOUR
T1 - Rat liver pp49, a protein that forms complexes with protein kinase CK2, is composed of the β and the γ subunits of translation initiation factor eIF-2
AU - Gil, Carles
AU - Plana, Maria
AU - Riera, Marta
AU - Itarte, Emilio
PY - 1996/8/23
Y1 - 1996/8/23
N2 - Comparison of the amino acid sequences of two peptides derived from proteolysis of rat liver pp49 identified it as composed of the β-subunit and the γ-subunit of eukaryotic initiation factor-2 (eIF-2). Partial purification of rat liver eIF-2 showed that its trimeric form (αβγ) co-eluted with protein kinase CK2. Heat-inactivated preparations of the trimeric form of eIF-2 inhibited CK2, increasing its Km for β-casein, as observed with pp49. The form of eIF-2 that lacks the β-subunit had no effect on CK2. These data indicate that the βγ subunits of eIF-2 may complex with CK2 and modulate its activity.
AB - Comparison of the amino acid sequences of two peptides derived from proteolysis of rat liver pp49 identified it as composed of the β-subunit and the γ-subunit of eukaryotic initiation factor-2 (eIF-2). Partial purification of rat liver eIF-2 showed that its trimeric form (αβγ) co-eluted with protein kinase CK2. Heat-inactivated preparations of the trimeric form of eIF-2 inhibited CK2, increasing its Km for β-casein, as observed with pp49. The form of eIF-2 that lacks the β-subunit had no effect on CK2. These data indicate that the βγ subunits of eIF-2 may complex with CK2 and modulate its activity.
U2 - 10.1006/bbrc.1996.1293
DO - 10.1006/bbrc.1996.1293
M3 - Article
SN - 0006-291X
VL - 225
SP - 1052
EP - 1057
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -