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Abstract
The front-face fluorescence spectroscopy technique was used to establish a rapid prediction model of riboflavin concentration in milk without prior sample preparation. The prediction model developed was then compared with two conventional high performance liquid chromatography (HPLC)-based quantification methods. The method of standard addition allowed detecting a linear correlation between fluorescence intensity and riboflavin concentration in 12% (w/w) reconstituted low-heat milk powder. Validation of the model yielded an R2 of 0.99 with a standard error of prediction of 0.13 mg/L. The results suggest a potential use of front-face fluorescence spectroscopy as a simple method for off- and in-line determinations of riboflavin in milk.
Original language | American English |
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Article number | 6 |
Number of pages | 6 |
Journal | Foods |
Volume | 9 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2020 |
Keywords
- Front-face fluorescence
- Milk
- Prediction
- Rapid quantification
- Riboflavin
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Dive into the research topics of 'Rapid quantification of riboflavin in milk by front-face fluorescence spectroscopy: A preliminary study'. Together they form a unique fingerprint.Projects
- 1 Finished
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APLICACION DE INDICADORES NATIVOS DE FLUORESCENCIA PARA CUANTIFICACION RAPIDA DE DAÑO TERMICO DURANTE EL PROCESADO DE LECHE
Castillo Zambudio, M. (Principal Investigator), Gallardo, J. J. (Investigator), Saldo Periago, J. (Investigator) & Zamora Viladomiu, A. (Investigator)
Spanish Ministry of Economy and Competitiveness (MINECO)
1/01/13 → 31/12/15
Project: Research Projects and Other Grants