Rapid quantification of riboflavin in milk by front-face fluorescence spectroscopy: A preliminary study

Ulises Alvarado, Anna Zamora, Jinfang Liu, Jordi Saldo, Manuel Castillo*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

The front-face fluorescence spectroscopy technique was used to establish a rapid prediction model of riboflavin concentration in milk without prior sample preparation. The prediction model developed was then compared with two conventional high performance liquid chromatography (HPLC)-based quantification methods. The method of standard addition allowed detecting a linear correlation between fluorescence intensity and riboflavin concentration in 12% (w/w) reconstituted low-heat milk powder. Validation of the model yielded an R2 of 0.99 with a standard error of prediction of 0.13 mg/L. The results suggest a potential use of front-face fluorescence spectroscopy as a simple method for off- and in-line determinations of riboflavin in milk.

Original languageAmerican English
Article number6
JournalFoods
Volume9
Issue number1
DOIs
Publication statusPublished - 2020

Keywords

  • Front-face fluorescence
  • Milk
  • Prediction
  • Rapid quantification
  • Riboflavin

Fingerprint Dive into the research topics of 'Rapid quantification of riboflavin in milk by front-face fluorescence spectroscopy: A preliminary study'. Together they form a unique fingerprint.

Cite this