Rapid fluorescent staining of histones in sodium dodecyl sulfate-polyacrylamide gels

Joan Ramon Daban, Anna M. Aragay

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

The increase in the fluorescence intensity of 1-anilinonaphthalene-8-sulfonate (ANS) produced by core histones is higher than that produced by very lysine-rich histones (H1 and H5). In the presence of the anionic detergent sodium dodecyl sulfate (SDS) the enhancement of ANS fluorescence caused by these two groups of histones is roughly the same, but much lower than that observed for core histones in the absence of this detergent. However, the increase of ANS fluorescence produced by histone-SDS complexes is high enough to use it for the staining of these proteins separated in SDS-polyacrylamide gels. Histone bands are stained with ANS after electrophoresis and visualized by transillumination of the gel with a uv light source. The method described in this work allows the rapid detection of less than 0.5 μg of histone per band. © 1984.
Original languageEnglish
Pages (from-to)223-228
JournalAnalytical Biochemistry
Volume138
Issue number1
DOIs
Publication statusPublished - 1 Jan 1984

Keywords

  • 1-anilinonaphthalene-8-sulfonate
  • fluorescent staining
  • gel staining
  • histones
  • hydrophobic probe
  • polyacrylamide gel electrophoresis

Fingerprint Dive into the research topics of 'Rapid fluorescent staining of histones in sodium dodecyl sulfate-polyacrylamide gels'. Together they form a unique fingerprint.

  • Cite this