Rapid detection of several mycobacterial species using a polymerase chain reaction reverse hybridisation assay

E. Padilla, J. Manterola, V. González, J. Lonca, J. Domínguez, N. Galí, V. Ausina, Juana Maria Lourdes Matas Andreu

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Abstract

The ability of a polymerase-chain-reaction-based technique combined with a reverse hybridisation line-probe assay to detect and identify eight of the most clinically significant mycobacterial species directly from cultures in liquid medium was evaluated. The line-probe assay allows simultaneous identification of eight different mycobacterial species. Ninety-seven mycobacterial strains belonging to 22 different species were evaluated. All strains were previously inoculated into MB/BacT bottles (Organon Teknika, USA). The sensitivity and specificity of the test when applied on positive MB/BacT liquid cultures containing isolates from mycobacterial species included as specific probes on the line-probe assay strip was 100% and 100%, respectively. These results further support the potential clinical usefulness of this technique in the diagnosis of mycobacterial infections.
Original languageEnglish
Pages (from-to)661-665
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume20
DOIs
Publication statusPublished - 22 Oct 2001

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    Padilla, E., Manterola, J., González, V., Lonca, J., Domínguez, J., Galí, N., Ausina, V., & Matas Andreu, J. M. L. (2001). Rapid detection of several mycobacterial species using a polymerase chain reaction reverse hybridisation assay. European Journal of Clinical Microbiology and Infectious Diseases, 20, 661-665. https://doi.org/10.1007/s100960100583