TY - JOUR
T1 - Rapid detection of legionella pneumophila in drinking water, based on filter immunoassay and chronoamperometric measurement
AU - Ezenarro, Josune J.
AU - Párraga-Niño, Noemí
AU - Sabrià, Miquel
AU - Del Campo, Fancisco Javier
AU - Muñoz-Pascual, Francesc Xavier
AU - Mas, Jordi
AU - Uria, Naroa
N1 - Funding Information:
Funding: The authors acknowledge financial support from the Spanish Ministry of Science, Innovation and University [BACSYS projects CTQ2014-54553-C3-1-R and C3-2R] and [RTI2018-101974-B-C22]. J.J.E. acknowledges financial support from the Catalan Industrial Doctorate program [2017-DI-051] and Waterologies S.L.
Publisher Copyright:
© 2020 by the authors.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/9
Y1 - 2020/9
N2 - Legionella is a pathogenic bacterium, ubiquitous in freshwater environments and able to colonise man-made water systems from which it can be transmitted to humans during outbreaks. The prevention of such outbreaks requires a fast, low cost, automated and often portable detection system. In this work, we present a combination of sample concentration, immunoassay detection, and measurement by chronoamperometry. A nitrocellulose microfiltration membrane is used as support for both the water sample concentration and the Legionella immunodetection. The horseradish peroxidase enzymatic label of the antibodies permits using the redox substrate 3,30,5,50-Tetramethylbenzidine to generate current changes proportional to the bacterial concentration present in drinking water. Carbon screen-printed electrodes are employed in the chronoamperometric measurements. Our system reduces the detection time: from the 10 days required by the conventional culture-based methods, to 2–3 h, which could be crucial to avoid outbreaks. Additionally, the system shows a linear response (R2 value of 0.99), being able to detect a range of Legionella concentrations between 101 and 104 cfu·mL−1 with a detection limit (LoD) of 4 cfu·mL−1
AB - Legionella is a pathogenic bacterium, ubiquitous in freshwater environments and able to colonise man-made water systems from which it can be transmitted to humans during outbreaks. The prevention of such outbreaks requires a fast, low cost, automated and often portable detection system. In this work, we present a combination of sample concentration, immunoassay detection, and measurement by chronoamperometry. A nitrocellulose microfiltration membrane is used as support for both the water sample concentration and the Legionella immunodetection. The horseradish peroxidase enzymatic label of the antibodies permits using the redox substrate 3,30,5,50-Tetramethylbenzidine to generate current changes proportional to the bacterial concentration present in drinking water. Carbon screen-printed electrodes are employed in the chronoamperometric measurements. Our system reduces the detection time: from the 10 days required by the conventional culture-based methods, to 2–3 h, which could be crucial to avoid outbreaks. Additionally, the system shows a linear response (R2 value of 0.99), being able to detect a range of Legionella concentrations between 101 and 104 cfu·mL−1 with a detection limit (LoD) of 4 cfu·mL−1
KW - Amperometry
KW - Immunodetection
KW - Legionella pneumophilla
KW - Preconcentration
UR - http://www.scopus.com/inward/record.url?scp=85089794257&partnerID=8YFLogxK
U2 - 10.3390/bios10090102
DO - 10.3390/bios10090102
M3 - Article
C2 - 32825468
AN - SCOPUS:85089794257
SN - 2079-6374
VL - 10
JO - Biosensors
JF - Biosensors
IS - 9
M1 - 102
ER -