Rapid chromatographic immunoassay study of brain PrPsc distribution in classical scrapie

Rosa Bolea; Enric Vidal; Belén Marín; Mercedes Márquez; Antonia Vargas; Martí Pumarola; Juan José Badiola

doi:10.1177/104063871002200115

Rapid chromatographic immunoassay study of brain PrPsc distribution in classical scrapie

Rosa Bolea, Enric Vidal, Belén Marín, Mercedes Márquez, Antonia Vargas, Martí Pumarola, Juan José Badiola

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Abstract

In the current study, a rapid chromatographic immunoassay submitted for registration in Europe was used to analyze PrPsc in 13 different areas of brain from 10 confirmed classical scrapie cases. The levels of PrPsc in the different areas of brain were plotted to draw a brain PrPsc distribution curve. This curve was compared with the brain PrPsc distribution curve obtained from immunoblotting and immunohisto- chemistry tests on the same samples. The distribution of PrPsc in different areas of the brain was similar, irrespective of the test applied, indicating that any of the 3 tests could be used for the characterization of classical cases of scrapie.

Original language	English
Pages (from-to)	82-85
Journal	Journal of Veterinary Diagnostic Investigation
Volume	22
Issue number	1
DOIs	https://doi.org/10.1177/104063871002200115
Publication status	Published - 1 Jan 2010

Keywords

Brain
Distribution curve
Immunoblotting
Immunohistochemistry
Prion protein
Rapid test
Scrapie

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10.1177/104063871002200115

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abstract = "In the current study, a rapid chromatographic immunoassay submitted for registration in Europe was used to analyze PrPsc in 13 different areas of brain from 10 confirmed classical scrapie cases. The levels of PrPsc in the different areas of brain were plotted to draw a brain PrPsc distribution curve. This curve was compared with the brain PrPsc distribution curve obtained from immunoblotting and immunohisto- chemistry tests on the same samples. The distribution of PrPsc in different areas of the brain was similar, irrespective of the test applied, indicating that any of the 3 tests could be used for the characterization of classical cases of scrapie.",

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AU - Bolea, Rosa

AU - Vidal, Enric

AU - Marín, Belén

AU - Márquez, Mercedes

AU - Vargas, Antonia

AU - Pumarola, Martí

AU - Badiola, Juan José

PY - 2010/1/1

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AB - In the current study, a rapid chromatographic immunoassay submitted for registration in Europe was used to analyze PrPsc in 13 different areas of brain from 10 confirmed classical scrapie cases. The levels of PrPsc in the different areas of brain were plotted to draw a brain PrPsc distribution curve. This curve was compared with the brain PrPsc distribution curve obtained from immunoblotting and immunohisto- chemistry tests on the same samples. The distribution of PrPsc in different areas of the brain was similar, irrespective of the test applied, indicating that any of the 3 tests could be used for the characterization of classical cases of scrapie.

KW - Brain

KW - Distribution curve

KW - Immunoblotting

KW - Immunohistochemistry

KW - Prion protein

KW - Rapid test

KW - Scrapie

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JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

IS - 1

ER -

Rapid chromatographic immunoassay study of brain PrPsc distribution in classical scrapie

Abstract

Keywords

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