Quasispecies dynamics in hepatitis C liver transplant recipients receiving grafts from hepatitis C virus infected donors

Sofía Pérez-Del-Pulgar, Josep Gregori, Francisco Rodríguez-Frías, Patricia González, Damir García-Cehic, Santseharay Ramírez, Rosario Casillas, Esteban Domingo, Juan I. Esteban, Xavier Forns, Josep Quer

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7 Citations (Scopus)

Abstract

© 2015 The Authors. The allocation of liver grafts from hepatitis C virus (HCV)-positive donors in HCV-infected liver transplant (LT) recipients leads to infection with two different viral populations. In a previous study, we examined quasispecies dynamics during reinfection by clonal sequencing, which did not allow an accurate characterization of coexistence and competition events. To overcome this limitation, here we used deep-sequencing analysis of a fragment of the HCV NS5B gene in six HCV-infected LT recipients who received HCV-infected grafts. Successive expansions and contractions of quasispecies complexity were observed, evolving in all cases towards a more homogeneous population. The population that became dominant was the one displaying the highest mutant spectrum complexity. In four patients, coexistence of minority mutants, derived from the donor or the recipient, were detected. In conclusion, our study shows that, during reinfection with a different HCV strain in LT recipients, the viral population with the highest diversity always becomes dominant.
Original languageEnglish
Pages (from-to)3493-3498
JournalJournal of General Virology
Volume96
Issue number12
DOIs
Publication statusPublished - 1 Dec 2015

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    Pérez-Del-Pulgar, S., Gregori, J., Rodríguez-Frías, F., González, P., García-Cehic, D., Ramírez, S., Casillas, R., Domingo, E., Esteban, J. I., Forns, X., & Quer, J. (2015). Quasispecies dynamics in hepatitis C liver transplant recipients receiving grafts from hepatitis C virus infected donors. Journal of General Virology, 96(12), 3493-3498. https://doi.org/10.1099/jgv.0.000289