Abstract
© 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).
Original language | English |
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Pages (from-to) | 210-219 |
Journal | Biochemical Engineering Journal |
Volume | 91 |
DOIs | |
Publication status | Published - 5 Oct 2014 |
Keywords
- E. coli
- IPTG
- Inducer transport
- Lac permeases
- Mathematical model