Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

Daniel Calleja, Alfred Fernández-Castañé, Martina Pasini, Carles de Mas, Josep López-Santín

    Research output: Contribution to journalArticleResearchpeer-review

    1 Citation (Scopus)

    Abstract

    © 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).
    Original languageEnglish
    Pages (from-to)210-219
    JournalBiochemical Engineering Journal
    Volume91
    DOIs
    Publication statusPublished - 5 Oct 2014

    Keywords

    • E. coli
    • IPTG
    • Inducer transport
    • Lac permeases
    • Mathematical model

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