Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

Daniel Calleja, Alfred Fernández-Castañé, Martina Pasini, Carles de Mas, Josep López-Santín

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Abstract

© 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).
Original languageEnglish
Pages (from-to)210-219
JournalBiochemical Engineering Journal
Volume91
DOIs
Publication statusPublished - 5 Oct 2014

Keywords

  • E. coli
  • IPTG
  • Inducer transport
  • Lac permeases
  • Mathematical model

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    Calleja, D., Fernández-Castañé, A., Pasini, M., de Mas, C., & López-Santín, J. (2014). Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli. Biochemical Engineering Journal, 91, 210-219. https://doi.org/10.1016/j.bej.2014.08.017