Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

Daniel Calleja; Alfred Fernández-Castañé; Martina Pasini; Carles de Mas; Josep López-Santín

doi:https://doi.org/10.1016/j.bej.2014.08.017

Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

Daniel Calleja, Alfred Fernández-Castañé, Martina Pasini, Carles de Mas, Josep López-Santín

Graduate School

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

© 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).

Original language	English
Pages (from-to)	210-219
Journal	Biochemical Engineering Journal
Volume	91
DOIs	https://doi.org/10.1016/j.bej.2014.08.017
Publication status	Published - 5 Oct 2014

Keywords

E. coli
IPTG
Inducer transport
Lac permeases
Mathematical model

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Calleja, D., Fernández-Castañé, A., Pasini, M., de Mas, C., & López-Santín, J. (2014). Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli. Biochemical Engineering Journal, 91, 210-219. https://doi.org/10.1016/j.bej.2014.08.017

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title = "Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli",

abstract = "{\textcopyright} 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).",

keywords = "E. coli, IPTG, Inducer transport, Lac permeases, Mathematical model",

author = "Daniel Calleja and Alfred Fern{\'a}ndez-Casta{\~n}{\'e} and Martina Pasini and {de Mas}, Carles and Josep L{\'o}pez-Sant{\'i}n",

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T1 - Quantitative modeling of inducer transport in fed-batch cultures of Escherichia coli

AU - Calleja, Daniel

AU - Fernández-Castañé, Alfred

AU - Pasini, Martina

AU - de Mas, Carles

AU - López-Santín, Josep

PY - 2014/10/5

Y1 - 2014/10/5

N2 - © 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).

AB - © 2014 Elsevier B.V. All rights reserved. An unsteady, unstructured, unsegregated and based on first principles mathematical model has been proposed to describe IPTG (isopropyl-β-. d-tiogalactopiranoside) transport in induced fed-batch cultures of E. coli M15 δ. glyA [pQEαβrham] [pREP4] producing rhamnulose 1-phosphate aldolase (RhuA). The model predicts extracellular and intracellular IPTG concentration. Experimental extracellular IPTG concentrations under different operational conditions were obtained by HPLC-MS analysis. These experimental data were used to fit the parameters of the model. The model was also able to predict the experimental behavior of two different E. coli strains producing fuculose 1-phosphate aldolase (FucA). IPTG transport to cells was the contribution of three processes: a diffusion process, and two active processes (one non-specific and another specific).

KW - E. coli

KW - IPTG

KW - Inducer transport

KW - Lac permeases

KW - Mathematical model

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DO - https://doi.org/10.1016/j.bej.2014.08.017

M3 - Article

VL - 91

SP - 210

EP - 219

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

SN - 1369-703X

ER -