Quantitative in-depth profiling of the postsynaptic density proteome to understand the molecular mechanisms governing synaptic physiology and pathology

Rita Reig-Viader, Àlex Bayés

Research output: Chapter in BookChapterResearchpeer-review

1 Citation (Scopus)

Abstract

© 2017, Springer Science+Business Media LLC. The postsynaptic density (PSD) is a very large protein complex found in excitatory synapses, the most abundant synaptic type in the brain. PSDs contain key proteins to postsynaptic functioning, being closely related to synaptic plasticity and underlying high cognitive abilities such as learning and memory. Biochemical isolation of PSDs has allowed for their profiling by mass spectrometry-based proteomics, revealing it as a highly complex structure containing over 2000 proteins. Genetic studies have found many genes expressed at the PSD as causing neurological as well as mental and behavioral disorders. Understanding the proteomic architecture and dynamics of the PSD is not only key to understand normal brain function but also highly incapacitating human conditions. Here we present easy and robust protocols to isolate PSDs from very little amounts of brain tissue and a mass spectrometry proteomics procedure that allows to identify and quantify over 2000 different PSD proteins in a routinely fashion.
Original languageEnglish
Title of host publicationNeuromethods
Pages255-280
Number of pages25
Volume127
ISBN (Electronic)1940-6045
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Biochemical fractionation
  • Label-free quantification
  • Mass spectrometry
  • Postsynaptic density
  • Proteomics

Fingerprint Dive into the research topics of 'Quantitative in-depth profiling of the postsynaptic density proteome to understand the molecular mechanisms governing synaptic physiology and pathology'. Together they form a unique fingerprint.

Cite this