Quantitative determination of aflatoxin B<inf>1</inf> in chick liver

Y. Espada, R. Guitart, M. Arboix

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    3 Citations (Scopus)


    A method for the rapid and quantitative determination of anatoxin Bi from small quantities of liver, around 1-2 g, is described. The extraction procedure involves acidification to pH 2 of the aqueous liver homogenates, extraction with chloroform: acetone and HPLC-fiuorimetric detection after derivatization with trifluoroacetic acid. Quantitative recovery of aflatoxin Bi from chick liver was achieved and detection at levels of 0-2-1 ppb was proved feasible. The aflatoxin Bi concentration in chick liver after oral administration is also shown. © 1991 Taylor & Francis Group, LLC.
    Original languageEnglish
    Pages (from-to)163-169
    JournalFood Additives and Contaminants
    Publication statusPublished - 1 Jan 1991


    • Aflatoxin B 1
    • Chick liver
    • One-step extraction procedure
    • RP-HPLC


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