Quantitation of the active compound and major excipients in a pharmaceutical formulation by near infrared diffuse reflectance spectroscopy with fibre optical probe

M. Blanco, J. Coello, H. Iturriaga, S. Maspoch, C. De La Pezuela

Research output: Contribution to journalArticleResearchpeer-review

57 Citations (Scopus)

Abstract

A new method for analyses of a commercial pharmaceutical formulation based on near infrared diffuse reflectance spectroscopy (NIRRS) with fibre optical probe and using partial least-squares regression (PLS) for multivariate calibration is proposed. Analyses include the determination of the active compound and major excipients. The influence of the spectral mode and wavelength range used are studied. Satisfactory predictive results for production samples require calibration with laboratory-made samples covering the concentration range involved in the manufacturing process, as well as samples from various production batches, which introduce the variation sources inherent in such a process. The number of production samples to be used for calibration in order to ensure correct prediction of new production samples is discussed.
Original languageEnglish
Pages (from-to)147-156
JournalAnalytica Chimica Acta
Volume333
Issue number1-2
DOIs
Publication statusPublished - 30 Oct 1996

Keywords

  • Fibre optical probe
  • Near infrared spectroscopy
  • Pharmaceutical

Fingerprint Dive into the research topics of 'Quantitation of the active compound and major excipients in a pharmaceutical formulation by near infrared diffuse reflectance spectroscopy with fibre optical probe'. Together they form a unique fingerprint.

Cite this