Quantification of amphetamine plasma concentrations by gas chromatography coupled to mass spectrometry

Nieves Pizarro, Jordi Ortuño, Jordi Segura, Magí Farré, Marta Mas, Jordi Camí, Rafael De La Torre

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

We developed a fast and sensitive method for identification and quantification of plasma concentrations of amphetamine using gas chromatography with mass spectrometry detection (GC-MS). Amphetamine-d8 served as internal standard. The method involves a single extraction procedure and an easy treatment of the samples that allowed no losses during the evaporation process. Derivatisation of amphetamine with N-methyl- bis(trifluoroacetamide), a potent acylating agent, provides many advantages to the method compared with common derivatisation reactions usually used for amphetamines. The limits of detection and quantification following this method were 0.43 and 1.42 ng/ml, respectively. The assay has been successfully employed in the quantification of amphetamine in plasma samples from healthy volunteers at four different doses.
Original languageEnglish
Pages (from-to)739-747
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume21
Issue number4
DOIs
Publication statusPublished - 1 Dec 1999

Keywords

  • Amphetamine
  • Gas Chromatography-Mass Spectrometry
  • Pharmacokinetics

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