Quantification of 3,4-methylenedioxymetamphetamine and its metabolites in plasma and urine by gas chromatography with nitrogen-phosphorus detection

Jordi Ortuño, Nieves Pizarro, Magí Farré, Marta Mas, Jordi Segura, Jordi Camí, Rudolf Brenneisen, Rafael De La Torre

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Abstract

A gas chromatographic method with nitrogen-phosphorus detection involving a solid-liquid extraction phase was developed and validated for the simultaneous quantification of 3,4-methylenedioxymethamphetamine (MDMA) and 3,4-methylenedioxyamphetamine (MDA) in plasma. A modification of this method was validated for the analysis of MDMA, MDA, 4-hydroxy-3-methoxymethamphetamine (HMMA) and, 4-hydroxy-3-methoxyamphetamine (HMA) in urine. Under the analytical conditions described, the limits of detection in plasma and urine were less than 1.6 μg/l and 47 μg/l, respectively, for all the compounds studied. Good linearity was observed in the concentration range evaluated in plasma (5-400 μg/l) and urine (100-2000 μg/l) for all compounds tested. The recoveries obtained from plasma were 85.1% and 91.6% for MDMA and MDA, respectively. Urine recoveries were higher than 90% for MDMA and MDA, 74% for HMMA, and 64% for HMA. Methods have been successfully used in the assessment of plasma and urine concentrations of MDMA and its main metabolites in samples from clinical studies in healthy volunteers. Copyright (C) 1999 Elsevier Science B.V.
Original languageEnglish
Pages (from-to)221-232
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume723
Issue number1-2
DOIs
Publication statusPublished - 19 Feb 1999

Keywords

  • 3,4-Methylenedioxyamphetamine
  • 3,4-Methylenedioxymethamphetamine

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    Ortuño, J., Pizarro, N., Farré, M., Mas, M., Segura, J., Camí, J., Brenneisen, R., & De La Torre, R. (1999). Quantification of 3,4-methylenedioxymetamphetamine and its metabolites in plasma and urine by gas chromatography with nitrogen-phosphorus detection. Journal of Chromatography B: Biomedical Sciences and Applications, 723(1-2), 221-232. https://doi.org/10.1016/S0378-4347(98)00506-4