Pyrosequencing for rapid detection of Mycobacterium tuberculosis second-line drugs and ethambutol resistance

Alicia Lacoma, Barbara Molina-Moya, Cristina Prat, Edita Pimkina, Jessica Diaz, Andriy Dudnyk, Nerea García-Sierra, Lucía Haba, Jose Maldonado, Sofia Samper, Juan Ruiz-Manzano, Vicente Ausina, Jose Dominguez

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

© 2015 Elsevier Inc.. The aim of this work was to study the diagnostic accuracy of pyrosequencing to detect resistance to fluoroquinolones, kanamycin, amikacin, capreomycin, and ethambutol (EMB) in Mycobacterium tuberculosis clinical strains. One hundred four clinical isolates previously characterized by BACTEC 460TB/MGIT 960 were included. Specific mutations were targeted in gyrA, rrs, eis promoter, and embB. When there was a discordant result between BACTEC and pyrosequencing, Genotype MTBDR. sl (Hain Lifescience, Nehren, Germany) was performed. Sensitivity and specificity of pyrosequencing were 70.6% and 100%, respectively, for fluoroquinolones; 93.3% and 81.7%, respectively, for kanamycin; 94.1% and 95.9%, respectively, for amikacin; 90.0% and 100%, respectively, for capreomycin; and 64.8% and 87.8%, respectively, for EMB. This study shows that pyrosequencing may be a useful tool for making early decisions regarding second-line drugs and EMB resistance. However, for a correct management of patients with suspected extensively drug-resistant tuberculosis, susceptibility results obtained by molecular methods should be confirmed by a phenotypic method.
Original languageEnglish
Pages (from-to)263-269
JournalDiagnostic Microbiology and Infectious Disease
Volume83
Issue number3
DOIs
Publication statusPublished - 1 Nov 2015

Keywords

  • Ethambutol
  • Extensively drug-resistant tuberculosis
  • Fluoroquinolones
  • Injectable drugs
  • Molecular diagnostic testing
  • Tuberculosis

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