Purification of human adenosine deaminase for the preparation of a reference material

Alexandre Bota, F. Javier Gella, Francesca Canalias

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7 Citations (Scopus)

Abstract

The goal was to optimise a purification procedure of adenosine deaminase from human erythrocytes for the preparation of a European Reference Material. Adenosine deaminase was purified from human erythrocytes with a specific activity of 4.46 μkat/mg of protein and a catalytic concentration of 133 μkat/l. The isolation and purification procedure involved ion-exchange chromatography (STREAMLINE(TM) DEAE), and two purine riboside affinity chromatographies. The purified enzyme exhibits a single band in SDS-PAGE with a molecular weight of 41 600 g/mol, and three bands in PAGE, isoelectric focusing and two-dimensional electrophoresis with pI 4.7, 4.85 and 5.0. © 2000 Elsevier Science B.V.
Original languageEnglish
Pages (from-to)237-244
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume737
Issue number1-2
DOIs
Publication statusPublished - 14 Jan 2000

Keywords

  • Adenosine deaminase
  • Enzymes
  • Purification

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