Purification and characterization of streptolysin o from Streptococcus pyogenes

Francesca Canalias, Jordi Viver, Jorge Beleta, Francesc Gonzalez-Sastre, F. Javier Gella

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4 Citations (Scopus)

Abstract

1. 1. Streptolysin O, an exotoxin produced by group A β-hemolytic streptococci, has been purified from Streptococcus pyogenes culture supernatants. 2. 2. The isolation and purification procedure involved ammonium sulphate and polyethylene glycol precipitations, and ion-exchange chromatographies on CM-Sepharose and Mono Q. 3. 3. The proposed procedure introduces two ion-exchange chromatography steps making the purification process simpler and, especially, more reproducible than other described protocols. 4. 4. The purified Streptolysin O was hemolytically active, had a specific activity of 415,000 HU/mg, an optimum pH of 7.0, a relative molecular mass of 60,100 and an isoelectric pH of 7.5. © 1992.
Original languageEnglish
Pages (from-to)1073-1079
JournalInternational Journal of Biochemistry
Volume24
DOIs
Publication statusPublished - 1 Jan 1992

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