TY - JOUR
T1 - Purification and characterization of streptolysin o from Streptococcus pyogenes
AU - Canalias, Francesca
AU - Viver, Jordi
AU - Beleta, Jorge
AU - Gonzalez-Sastre, Francesc
AU - Gella, F. Javier
PY - 1992/1/1
Y1 - 1992/1/1
N2 - 1. 1. Streptolysin O, an exotoxin produced by group A β-hemolytic streptococci, has been purified from Streptococcus pyogenes culture supernatants. 2. 2. The isolation and purification procedure involved ammonium sulphate and polyethylene glycol precipitations, and ion-exchange chromatographies on CM-Sepharose and Mono Q. 3. 3. The proposed procedure introduces two ion-exchange chromatography steps making the purification process simpler and, especially, more reproducible than other described protocols. 4. 4. The purified Streptolysin O was hemolytically active, had a specific activity of 415,000 HU/mg, an optimum pH of 7.0, a relative molecular mass of 60,100 and an isoelectric pH of 7.5. © 1992.
AB - 1. 1. Streptolysin O, an exotoxin produced by group A β-hemolytic streptococci, has been purified from Streptococcus pyogenes culture supernatants. 2. 2. The isolation and purification procedure involved ammonium sulphate and polyethylene glycol precipitations, and ion-exchange chromatographies on CM-Sepharose and Mono Q. 3. 3. The proposed procedure introduces two ion-exchange chromatography steps making the purification process simpler and, especially, more reproducible than other described protocols. 4. 4. The purified Streptolysin O was hemolytically active, had a specific activity of 415,000 HU/mg, an optimum pH of 7.0, a relative molecular mass of 60,100 and an isoelectric pH of 7.5. © 1992.
U2 - https://doi.org/10.1016/0020-711X(92)90376-C
DO - https://doi.org/10.1016/0020-711X(92)90376-C
M3 - Article
SN - 0020-711X
VL - 24
SP - 1073
EP - 1079
JO - International Journal of Biochemistry
JF - International Journal of Biochemistry
ER -