TY - JOUR
T1 - Proteomics study of human cord blood reticulocyte-derived exosomes
AU - Díaz-Varela, Míriam
AU - de Menezes-Neto, Armando
AU - Perez-Zsolt, Daniel
AU - Gámez-Valero, Ana
AU - Seguí-Barber, Joan
AU - Izquierdo-Useros, Nuria
AU - Martinez-Picado, Javier
AU - Fernández-Becerra, Carmen
AU - del Portillo, Hernando A.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - © 2018, The Author(s). Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex (HuRex). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, most of them related to exosomes as revealed by gene ontology over-representation analysis and include multiple transporters as well as proteins involved in exosome biogenesis and erythrocytic disorders. Immunoelectron microscopy validated the presence of the transferrin receptor. Moreover, functional assays demonstrated active capture of HuRex by mature dendritic cells. As only seven proteins have been previously associated with HuRex, this resource will facilitate studies on the role of human reticulocyte-derived exosomes in normal and pathological conditions affecting erythropoiesis.
AB - © 2018, The Author(s). Reticulocyte-derived exosomes (Rex), extracellular vesicles of endocytic origin, were initially discovered as a cargo-disposal mechanism of obsolete proteins in the maturation of reticulocytes into erythrocytes. In this work, we present the first mass spectrometry-based proteomics of human Rex (HuRex). HuRex were isolated from cultures of human reticulocyte-enriched cord blood using different culture conditions and exosome isolation methods. The newly described proteome consists of 367 proteins, most of them related to exosomes as revealed by gene ontology over-representation analysis and include multiple transporters as well as proteins involved in exosome biogenesis and erythrocytic disorders. Immunoelectron microscopy validated the presence of the transferrin receptor. Moreover, functional assays demonstrated active capture of HuRex by mature dendritic cells. As only seven proteins have been previously associated with HuRex, this resource will facilitate studies on the role of human reticulocyte-derived exosomes in normal and pathological conditions affecting erythropoiesis.
U2 - 10.1038/s41598-018-32386-2
DO - 10.1038/s41598-018-32386-2
M3 - Article
C2 - 30232403
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 14046
ER -