Proteomics identification of differentially expressed proteins in the muscle of dysferlin myopathy patients

Carolina De la Torre, Isabel Illa, Georgine Faulkner, Laura Soria, Rene Robles-Cedeño, Raul Dominguez-Perles, Noemi De Luna, Eduard Gallardo

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11 Citations (Scopus)

Abstract

The muscular dystrophies are a large and heterogeneous group of neuromuscular disorders that can be classified according to the mode of inheritance, the clinical phenotype and the molecular defect. To better understand the pathological mechanisms of dysferlin myopathy we compared the protein-expression pattern in the muscle biopsies of six patients with this disease with six patients with limb girdle muscular dystrophy 2A, five with facioscapulohumeral dystrophy and six normal control subjects. To investigate differences in the expression levels of skeletal muscle proteins we used 2-DE and MS. Western blot or immunohistochemistry confirmed relevant results. The study showed specific increase expression of proteins involved in fast-to-slow fiber type conversion (ankyrin repeat protein 2), type I predominance (phosphorylated forms of slow troponin T), sarcomere stabilization (actinin-associated LIM protein), protein ubiquitination (TRIM 72) and skeletal muscle differentiation (Rho-GDP-dissociation inhibitor ly-GDI) in dysferlin myopathy. As anticipated, we also found differential expression of proteins common to all the muscular dystrophies studied. This comparative proteomic analysis suggests that in dysferlin myopathy (i) the type I fiber predominance is an active process of fiber type conversion rather than a selective loss of type II fibers and (ii) the dysregulation of proteins involved in muscle differentiation further confirms the role of dysferlin in this process. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)486-497
JournalProteomics - Clinical Applications
Volume3
Issue number4
DOIs
Publication statusPublished - 14 May 2009

Keywords

  • 2-DE
  • Dysferlin
  • Facioscapulohumerial muscular dystrophy
  • Limb girdle muscular dystrophy 2A
  • Muscular dystrophies

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