Proteome of the bacterium Mycoplasma penetrans

Mario Ferrer-Navarro, Antonio Gómez, Oscar Yanes, Raquel Planell, Francesc Xavier Avilés, Jaume Piñol, Josep Antoni Pérez Pons, Enrique Querol

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

A proteome map of Mycoplasma penetrans has been constructed using two-dimensional gel electrophoresis in combination with mass spectrometry (MS). Mycoplasma penetrans infects the urogenital and respiratory tracts of humans. A total of 207 spots were characterized with MS and, in comparing the experimental data with the DNA sequence-derived predictions, it was possible to assign these 207 spots to 153 genes. The Pro-Q Diamond phosphoprotein dye technology was used for the fluorescent detection of 26 phosphoproteins in the 4-7 pH range. © 2006 American Chemical Society.
Original languageEnglish
Pages (from-to)688-694
JournalJournal of Proteome Research
Volume5
Issue number3
DOIs
Publication statusPublished - 1 Mar 2006

Keywords

  • Genome annotation
  • Mass spectrometry
  • Phosphoproteome
  • Proteomics
  • Two-dimensional gel electrophoresis

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