Proteome of the bacterium Mycoplasma penetrans

Mario Ferrer-Navarro; Antonio Gómez; Oscar Yanes; Raquel Planell; Francesc Xavier Avilés; Jaume Piñol; Josep Antoni Pérez Pons; Enrique Querol

doi:10.1021/pr050340p

Proteome of the bacterium Mycoplasma penetrans

Mario Ferrer-Navarro, Antonio Gómez, Oscar Yanes, Raquel Planell, Francesc Xavier Avilés, Jaume Piñol, Josep Antoni Pérez Pons, Enrique Querol

Research output: Contribution to journal › Article › Research › peer-review

20 Citations (Scopus)

Abstract

A proteome map of Mycoplasma penetrans has been constructed using two-dimensional gel electrophoresis in combination with mass spectrometry (MS). Mycoplasma penetrans infects the urogenital and respiratory tracts of humans. A total of 207 spots were characterized with MS and, in comparing the experimental data with the DNA sequence-derived predictions, it was possible to assign these 207 spots to 153 genes. The Pro-Q Diamond phosphoprotein dye technology was used for the fluorescent detection of 26 phosphoproteins in the 4-7 pH range. © 2006 American Chemical Society.

Original language	English
Pages (from-to)	688-694
Journal	Journal of Proteome Research
Volume	5
Issue number	3
DOIs	https://doi.org/10.1021/pr050340p
Publication status	Published - 1 Mar 2006

Keywords

Genome annotation
Mass spectrometry
Phosphoproteome
Proteomics
Two-dimensional gel electrophoresis

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10.1021/pr050340p

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title = "Proteome of the bacterium Mycoplasma penetrans",

abstract = "A proteome map of Mycoplasma penetrans has been constructed using two-dimensional gel electrophoresis in combination with mass spectrometry (MS). Mycoplasma penetrans infects the urogenital and respiratory tracts of humans. A total of 207 spots were characterized with MS and, in comparing the experimental data with the DNA sequence-derived predictions, it was possible to assign these 207 spots to 153 genes. The Pro-Q Diamond phosphoprotein dye technology was used for the fluorescent detection of 26 phosphoproteins in the 4-7 pH range. {\textcopyright} 2006 American Chemical Society.",

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author = "Mario Ferrer-Navarro and Antonio G{\'o}mez and Oscar Yanes and Raquel Planell and Avil{\'e}s, {Francesc Xavier} and Jaume Pi{\~n}ol and {P{\'e}rez Pons}, {Josep Antoni} and Enrique Querol",

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T1 - Proteome of the bacterium Mycoplasma penetrans

AU - Ferrer-Navarro, Mario

AU - Gómez, Antonio

AU - Yanes, Oscar

AU - Planell, Raquel

AU - Avilés, Francesc Xavier

AU - Piñol, Jaume

AU - Pérez Pons, Josep Antoni

AU - Querol, Enrique

PY - 2006/3/1

Y1 - 2006/3/1

N2 - A proteome map of Mycoplasma penetrans has been constructed using two-dimensional gel electrophoresis in combination with mass spectrometry (MS). Mycoplasma penetrans infects the urogenital and respiratory tracts of humans. A total of 207 spots were characterized with MS and, in comparing the experimental data with the DNA sequence-derived predictions, it was possible to assign these 207 spots to 153 genes. The Pro-Q Diamond phosphoprotein dye technology was used for the fluorescent detection of 26 phosphoproteins in the 4-7 pH range. © 2006 American Chemical Society.

AB - A proteome map of Mycoplasma penetrans has been constructed using two-dimensional gel electrophoresis in combination with mass spectrometry (MS). Mycoplasma penetrans infects the urogenital and respiratory tracts of humans. A total of 207 spots were characterized with MS and, in comparing the experimental data with the DNA sequence-derived predictions, it was possible to assign these 207 spots to 153 genes. The Pro-Q Diamond phosphoprotein dye technology was used for the fluorescent detection of 26 phosphoproteins in the 4-7 pH range. © 2006 American Chemical Society.

KW - Genome annotation

KW - Mass spectrometry

KW - Phosphoproteome

KW - Proteomics

KW - Two-dimensional gel electrophoresis

U2 - 10.1021/pr050340p

DO - 10.1021/pr050340p

M3 - Article

VL - 5

SP - 688

EP - 694

IS - 3

ER -

Proteome of the bacterium Mycoplasma penetrans

Abstract

Keywords

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