Protein nanoparticles are nontoxic, tuneable cell stressors

Marianna Teixeira De Pinho Favaro, Laura Sánchez-García, Alejandro Sánchez-Chardi, Mónica Roldán, Ugutz Unzueta, Naroa Serna, Olivia Cano-Garrido, Adriano Rodrigues Azzoni, Neus Ferrer-Miralles, Antonio Villaverde, Esther Vázquez

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

© 2018 2018 Future Medicine Ltd. Aim: Nanoparticle-cell interactions can promote cell toxicity and stimulate particular behavioral patterns, but cell responses to protein nanomaterials have been poorly studied. Results: By repositioning oligomerization domains in a simple, modular self-assembling protein platform, we have generated closely related but distinguishable homomeric nanoparticles. Composed by building blocks with modular domains arranged in different order, they share amino acid composition. These materials, once exposed to cultured cells, are differentially internalized in absence of toxicity and trigger distinctive cell adaptive responses, monitored by the emission of tubular filopodia and enhanced drug sensitivity. Conclusion: The capability to rapidly modulate such cell responses by conventional protein engineering reveals protein nanoparticles as tuneable, versatile and potent cell stressors for cell-targeted conditioning.
Original languageEnglish
Pages (from-to)255-268
JournalNanomedicine
Volume13
Issue number3
DOIs
Publication statusPublished - 1 Feb 2018

Keywords

  • molecular therapy
  • nanoparticles
  • recombinant proteins
  • self-assembling
  • stressor

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