Protein kinase CK2 is altered in insulin-resistant genetically obese (fa/fa) rats

Nerea Roher; Francesc Miró; Marta José; Ramon Trujillo; Maria Plana; Emilio Itarte

doi:https://doi.org/10.1016/S0014-5793(98)01230-7

Protein kinase CK2 is altered in insulin-resistant genetically obese (fa/fa) rats

Nerea Roher, Francesc Miró, Marta José, Ramon Trujillo, Maria Plana, Emilio Itarte

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

3 Citations (Scopus)

Abstract

Hepatic insulin receptor levels in 6-week-old obese (fa/fa) rats were about 2-fold lower than those from lean (Fa/-) rats, which agrees with their insulin-resistant state. Nuclear protein kinase CK2 activity and protein content in livers from obese (fa/fa) rats were similar to those of lean (Fa/-) animals but the cytosolic levels were reduced to half, due to a decrease in the 39-kDa catalytic subunit. Marked increases in activity, due to rises in the 44-kDa and 39-kDa catalytic subunits, were seen in the 16 000xg sediments (M1) from insulin-resistant rats, with moderate changes in the 100 000xg sediments (M2). The increase in CK2 binding to M1 did not require increases in the molecular chaperone grp94, which was unaltered in insulin-resistant rats. Copyright (C) 1998 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	211-215
Journal	FEBS Letters
Volume	437
DOIs	https://doi.org/10.1016/S0014-5793(98)01230-7
Publication status	Published - 23 Oct 1998

Keywords

Grp94
Insulin receptor
Insulin resistance
Obese (fa/fa) rat
Protein kinase CK2
Rat liver

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title = "Protein kinase CK2 is altered in insulin-resistant genetically obese (fa/fa) rats",

abstract = "Hepatic insulin receptor levels in 6-week-old obese (fa/fa) rats were about 2-fold lower than those from lean (Fa/-) rats, which agrees with their insulin-resistant state. Nuclear protein kinase CK2 activity and protein content in livers from obese (fa/fa) rats were similar to those of lean (Fa/-) animals but the cytosolic levels were reduced to half, due to a decrease in the 39-kDa catalytic subunit. Marked increases in activity, due to rises in the 44-kDa and 39-kDa catalytic subunits, were seen in the 16 000xg sediments (M1) from insulin-resistant rats, with moderate changes in the 100 000xg sediments (M2). The increase in CK2 binding to M1 did not require increases in the molecular chaperone grp94, which was unaltered in insulin-resistant rats. Copyright (C) 1998 Federation of European Biochemical Societies.",

keywords = "Grp94, Insulin receptor, Insulin resistance, Obese (fa/fa) rat, Protein kinase CK2, Rat liver",

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T1 - Protein kinase CK2 is altered in insulin-resistant genetically obese (fa/fa) rats

AU - Roher, Nerea

AU - Miró, Francesc

AU - José, Marta

AU - Trujillo, Ramon

AU - Plana, Maria

AU - Itarte, Emilio

PY - 1998/10/23

Y1 - 1998/10/23

N2 - Hepatic insulin receptor levels in 6-week-old obese (fa/fa) rats were about 2-fold lower than those from lean (Fa/-) rats, which agrees with their insulin-resistant state. Nuclear protein kinase CK2 activity and protein content in livers from obese (fa/fa) rats were similar to those of lean (Fa/-) animals but the cytosolic levels were reduced to half, due to a decrease in the 39-kDa catalytic subunit. Marked increases in activity, due to rises in the 44-kDa and 39-kDa catalytic subunits, were seen in the 16 000xg sediments (M1) from insulin-resistant rats, with moderate changes in the 100 000xg sediments (M2). The increase in CK2 binding to M1 did not require increases in the molecular chaperone grp94, which was unaltered in insulin-resistant rats. Copyright (C) 1998 Federation of European Biochemical Societies.

AB - Hepatic insulin receptor levels in 6-week-old obese (fa/fa) rats were about 2-fold lower than those from lean (Fa/-) rats, which agrees with their insulin-resistant state. Nuclear protein kinase CK2 activity and protein content in livers from obese (fa/fa) rats were similar to those of lean (Fa/-) animals but the cytosolic levels were reduced to half, due to a decrease in the 39-kDa catalytic subunit. Marked increases in activity, due to rises in the 44-kDa and 39-kDa catalytic subunits, were seen in the 16 000xg sediments (M1) from insulin-resistant rats, with moderate changes in the 100 000xg sediments (M2). The increase in CK2 binding to M1 did not require increases in the molecular chaperone grp94, which was unaltered in insulin-resistant rats. Copyright (C) 1998 Federation of European Biochemical Societies.

KW - Grp94

KW - Insulin receptor

KW - Insulin resistance

KW - Obese (fa/fa) rat

KW - Protein kinase CK2

KW - Rat liver

U2 - https://doi.org/10.1016/S0014-5793(98)01230-7

DO - https://doi.org/10.1016/S0014-5793(98)01230-7

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