Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release

Carles Gil, Anton Falqués, Eduard Sarró, Roger Cubí, Juan Blasi, José Aguilera, Emilio Itarte

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

In the present work we report the presence of protein kinase CK2 in lipid raft preparations from rat brain synaptosomes, obtained after detergent extraction and subsequent isolation of detergent-resistant membranes using sucrose gradient ultracentrifugation. Moreover, the phosphorylation of syntaxin-1 at Ser14, a specific CK2 target, has been detected in lipid rafts, as assessed by a phospho-specific antibody. Treatment with DMAT, a specific CK2 inhibitor, results in a decrease of syntaxin-1 Ser14 phosphorylation in lipid rafts, while the glutamate release from synaptosomes is enhanced. In conclusion, CK2 might control neurotransmitter release by acting on SNARE proteins attached to cholesterol-enriched microdomains. © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)414-420
JournalFEBS Letters
Volume585
Issue number2
DOIs
Publication statusPublished - 21 Jan 2011

Keywords

  • Cholesterol
  • Glutamate release
  • Lipid raft
  • Protein kinase CK2
  • Synaptosome
  • Syntaxin

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