Protein aggregation and soluble aggregate formation screened by a fast microdialysis assay

Verónica Toledo-Rubio, Esther Vazquez, Gemma Platas, Joan Domingo-Espín, Ugutz Unzueta, Elke Steinkamp, Elena García-Fruitós, Neus Ferrer-Miralles, Antonio Villaverde*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Protein aggregation is a major obstacle in recombinant protein production as it reduces the yield of soluble polypeptides. Also, the formation of aggregates occurring in the soluble fraction is more common than formerly expected, and the prevalence of these entities might significantly affect the average quality of the soluble protein species. Usually, the formation of soluble aggregates remains unperceived because analytical methods such as dynamic light scattering are not routinely applied as quality control procedures. The authors have developed a methodologically simple and fast procedure, based on microdialysis and image processing, that reveals the aggregation tendency of a given protein in a specific environment. Because they also show a good correlation between macroscopic aggregation and soluble aggregate formation, the microdialysis approach also permits an estimation of the occurrence of soluble aggregates.

Original languageAmerican English
Pages (from-to)453-457
Number of pages5
JournalJournal of Biomolecular Screening
Volume15
Issue number4
DOIs
Publication statusPublished - Apr 2010

Keywords

  • Dynamic light scattering
  • Nanoparticles
  • Protein aggregation
  • Proto-aggregates
  • Soluble aggregates

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