Protective effect of vitamin E in an animal model of LPS-induced inflammation

M. Mayorga, A. Iborra, S. Estany, Paz Martínez

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

Problem: Many sterility outcomes may be associated to the presence of an inflammatory response that would lead to an inability of the endometrium to support implantation and maintain viable embryos. We have established an animal model of inflammation in which the systemic administration of lipopolysaccharyde (LPS) results in a low embryo implantation rate. The purpose of this work was to investigate the effect of the inflammatory agent LPS on embryo viability and to verify the ability of vitamin E to modulate the inflammatory effect of LPS on embryo viability. Method of the study: For pre-implantation studies B6CBAF1 mice, which were intraperitoneally inoculated with LPS (4-10 mg/kg), were used. Mice were also treated with vitamin E (4-10 mg/kg) before or after LPS injection. Embryos were obtained from the oviduct after each treatment. Results: The LPS produces a decrease in the number of preimplantational embryos in a concentration dependent manner. The LPS effect can be partially reversed or prevented by vitamin E. Preliminary results show that inflammatory cytokines are secreted by intraperitoneal macrophages in LPS treated mice. Conclusion: Our results demonstrate the ability of vitamin E to avoid an inflammatory environment and to allow viability of embryos. © Blackwell Munksgaard, 2004.
Original languageEnglish
Pages (from-to)356-361
JournalAmerican Journal of Reproductive Immunology
Volume52
Issue number6
Publication statusPublished - 1 Dec 2004

Keywords

  • Embryo viability
  • Inflammatory response
  • Lipopolysaccharyde
  • Oxidative stress

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