Protection of spanish ibex (capra pyrenaica) against bluetongue virus serotypes 1 and 8 in a subclinical experimental infection

Cristina Lorca-Oró, Joan Pujols, Ignacio García-Bocanegra, Gregorio Mentaberre, José Enrique Granados, David Solanes, Paulino Fandos, Iván Galindo, Mariano Domingo, Santiago Lavín, Jorge Ramón López-Olvera

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8 Citations (Scopus)

Abstract

Many wild ruminants such as Spanish ibex (Capra pyrenaica) are susceptible to Bluetongue virus (BTV) infection, which causes disease mainly in domestic sheep and cattle. Outbreaks involving either BTV serotypes 1 (BTV-1) and 8 (BTV-8) are currently challenging Europe. Inclusion of wildlife vaccination among BTV control measures should be considered in certain species. In the present study, four out of fifteen seronegative Spanish ibexes were immunized with a single dose of inactivated vaccine against BTV-1, four against BTV-8 and seven ibexes were non vaccinated controls. Seven ibexes (four vaccinated and three controls) were inoculated with each BTV serotype. Antibody and IFN-gamma responses were evaluated until 28 days after inoculation (dpi). The vaccinated ibexes showed significant (P<0.05) neutralizing antibody levels after vaccination compared to non vaccinated ibexes. The non vaccinated ibexes remained seronegative until challenge and showed neutralizing antibodies from 7 dpi. BTV RNA was detected in the blood of non vaccinated ibexes from 2 to the end of the study (28 dpi) and in target tissue samples obtained at necropsy (8 and 28 dpi). BTV-1 was successfully isolated on cell culture from blood and target tissues of non vaccinated ibexes. Clinical signs were unapparent and no gross lesions were found at necropsy. Our results show for the first time that Spanish ibex is susceptible and asymptomatic to BTV infection and also that a single dose of vaccine prevents viraemia against BTV-1 and BTV-8 replication. © 2012 Lorca-Oró et al.
Original languageEnglish
Article numbere36380
JournalPLoS ONE
Volume7
Issue number5
DOIs
Publication statusPublished - 30 May 2012

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