Programmed death-ligand (PD-L1), epidermal growth factor (EGF), relaxin and metalloproteinase-3 (MMP3), potential biomarkers of malignancy in canine mammary neoplasia.

Makchit Galadima, Mariana Teles Pereira, Josep Pastor Milan, Javier Hernández Losa, Juan Enrique Rodriguez Gil, Maria Montserrat Rivera del Alamo*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

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Abstract

Gene expression has been suggested as a putative tool for prognosis and diagnosis in canine mammary neoplasia (CMNs). In the present study, 58 formalin-fixed paraffin-embedded (FFPE) paraffined canine mammary neoplasias from 27 different bitches were included. Thirty-seven tumours were classified as benign, whereas thirty-one were classified as different types of canine carcinoma. In addition, mammary samples from three healthy bitches were also included. The
gene expression for vascular endothelial growth factor-α (VEGFα), CD20, progesterone receptor (PGR), hyaluronidase-1 (HYAL-1), programmed death-ligand 1 (PD-L1), epidermal growth factor (EGF), relaxin (RLN2), and matrix metalloproteinase-3 (MMP3) was assessed through RT-qPCR. All the assessed genes yielded a higher expression in neoplastic mammary tissue than in healthy
tissue. All the evaluated genes were overexpressed in neoplastic mammary tissue, suggesting a role in the process of tumorigenesis. Moreover, PD-L1, EGF, relaxin, and MMP3 were significantly overexpressed in malignant CMNs compared to benign CMNs, suggesting they may be useful as malignancy biomarkers.
Translated title of the contribution"Programmed death-ligand" (PD-L1), "epidermal growth factor" (EGF), relaxina y metaloproteinasa-3 (MMP3), potenciales biomarcadores de malignidad in neoplasias mamarias caninas .
Original languageEnglish
Article number1170
Number of pages15
JournalInternational journal of molecular sciences (Online)
Volume25
Publication statusPublished - 18 Jan 2024

Keywords

  • canine mammary neoplasia
  • gene expression
  • malignancy biomarkers
  • programmed death-ligand 1
  • Epidermal growth factor
  • Relaxin
  • Matrix metalloprotease-3

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