Prognostic value of tumoral stem cells in glioblastoma: Immunohistochemical study of CD133 and HTERT expression

José Manuel Rute; Maria Martínez; Joan Gibert; Pilar Navarro; Beatriz Bellosillo; Francesc Alameda

Prognostic value of tumoral stem cells in glioblastoma: Immunohistochemical study of CD133 and HTERT expression

José Manuel Rute, Maria Martínez, Joan Gibert, Pilar Navarro, Beatriz Bellosillo, Francesc Alameda

Department of Morphological Sciences

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

© Science Printers and Publishers, Inc. OBJECTIVE: To study the prognostic value of CD133 and HTERT immunoexpression in glioblastoma (GB). GBs are the most frequent primary tumors in the central nervous system. According to the tumor stem cell (TSC) theory, the growth, relapse, and therapeutic resistance of GB would be determined by the presence of a TSC subpopulation. It has been proposed that TSCs can be identified by means of immunohistochemistry for CD133. Furthermore, the percentage of TSCs could be an independent factor of poor prognosis; however, some authors have shown that CD133 expression does not correlate with prognosis. TSCs may express other tumor markers, such as HTERT. STUDY DESIGN: We determined the percentage of CD133+ and HTERT+ tumor cells in 55 GBs using a tissue microarray. The data were correlated with clinical outcome. RESULTS: The increase in CD133 immunoexpression correlated with an increase in survival (p=0.034). HTERT expression alone was not found to be related to poor survival (p=0.385) but was linked to poor prognosis associated with CD133 expression (p=0.04). CONCLUSION: Our results reveal the increase in HTERT expression associated with augmented CD133 immunoexpression as a prognostic factor in GB.

Original language	English
Pages (from-to)	259-264
Journal	Analytical and Quantitative Cytopathology and Histopathology
Volume	39
Issue number	5
Publication status	Published - 1 Oct 2017

Keywords

CD133 antigen
Glioblastoma
HTERT
Prognosis
Tumor stem cells

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title = "Prognostic value of tumoral stem cells in glioblastoma: Immunohistochemical study of CD133 and HTERT expression",

abstract = "{\textcopyright} Science Printers and Publishers, Inc. OBJECTIVE: To study the prognostic value of CD133 and HTERT immunoexpression in glioblastoma (GB). GBs are the most frequent primary tumors in the central nervous system. According to the tumor stem cell (TSC) theory, the growth, relapse, and therapeutic resistance of GB would be determined by the presence of a TSC subpopulation. It has been proposed that TSCs can be identified by means of immunohistochemistry for CD133. Furthermore, the percentage of TSCs could be an independent factor of poor prognosis; however, some authors have shown that CD133 expression does not correlate with prognosis. TSCs may express other tumor markers, such as HTERT. STUDY DESIGN: We determined the percentage of CD133+ and HTERT+ tumor cells in 55 GBs using a tissue microarray. The data were correlated with clinical outcome. RESULTS: The increase in CD133 immunoexpression correlated with an increase in survival (p=0.034). HTERT expression alone was not found to be related to poor survival (p=0.385) but was linked to poor prognosis associated with CD133 expression (p=0.04). CONCLUSION: Our results reveal the increase in HTERT expression associated with augmented CD133 immunoexpression as a prognostic factor in GB.",

keywords = "CD133 antigen, Glioblastoma, HTERT, Prognosis, Tumor stem cells",

author = "Rute, {Jos{\'e} Manuel} and Maria Mart{\'i}nez and Joan Gibert and Pilar Navarro and Beatriz Bellosillo and Francesc Alameda",

year = "2017",

month = oct,

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language = "English",

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Prognostic value of tumoral stem cells in glioblastoma: Immunohistochemical study of CD133 and HTERT expression. / Rute, José Manuel; Martínez, Maria; Gibert, Joan; Navarro, Pilar; Bellosillo, Beatriz; Alameda, Francesc.

In: Analytical and Quantitative Cytopathology and Histopathology, Vol. 39, No. 5, 01.10.2017, p. 259-264.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Prognostic value of tumoral stem cells in glioblastoma: Immunohistochemical study of CD133 and HTERT expression

AU - Rute, José Manuel

AU - Martínez, Maria

AU - Gibert, Joan

AU - Navarro, Pilar

AU - Bellosillo, Beatriz

AU - Alameda, Francesc

PY - 2017/10/1

Y1 - 2017/10/1

N2 - © Science Printers and Publishers, Inc. OBJECTIVE: To study the prognostic value of CD133 and HTERT immunoexpression in glioblastoma (GB). GBs are the most frequent primary tumors in the central nervous system. According to the tumor stem cell (TSC) theory, the growth, relapse, and therapeutic resistance of GB would be determined by the presence of a TSC subpopulation. It has been proposed that TSCs can be identified by means of immunohistochemistry for CD133. Furthermore, the percentage of TSCs could be an independent factor of poor prognosis; however, some authors have shown that CD133 expression does not correlate with prognosis. TSCs may express other tumor markers, such as HTERT. STUDY DESIGN: We determined the percentage of CD133+ and HTERT+ tumor cells in 55 GBs using a tissue microarray. The data were correlated with clinical outcome. RESULTS: The increase in CD133 immunoexpression correlated with an increase in survival (p=0.034). HTERT expression alone was not found to be related to poor survival (p=0.385) but was linked to poor prognosis associated with CD133 expression (p=0.04). CONCLUSION: Our results reveal the increase in HTERT expression associated with augmented CD133 immunoexpression as a prognostic factor in GB.

AB - © Science Printers and Publishers, Inc. OBJECTIVE: To study the prognostic value of CD133 and HTERT immunoexpression in glioblastoma (GB). GBs are the most frequent primary tumors in the central nervous system. According to the tumor stem cell (TSC) theory, the growth, relapse, and therapeutic resistance of GB would be determined by the presence of a TSC subpopulation. It has been proposed that TSCs can be identified by means of immunohistochemistry for CD133. Furthermore, the percentage of TSCs could be an independent factor of poor prognosis; however, some authors have shown that CD133 expression does not correlate with prognosis. TSCs may express other tumor markers, such as HTERT. STUDY DESIGN: We determined the percentage of CD133+ and HTERT+ tumor cells in 55 GBs using a tissue microarray. The data were correlated with clinical outcome. RESULTS: The increase in CD133 immunoexpression correlated with an increase in survival (p=0.034). HTERT expression alone was not found to be related to poor survival (p=0.385) but was linked to poor prognosis associated with CD133 expression (p=0.04). CONCLUSION: Our results reveal the increase in HTERT expression associated with augmented CD133 immunoexpression as a prognostic factor in GB.

KW - CD133 antigen

KW - Glioblastoma

KW - HTERT

KW - Prognosis

KW - Tumor stem cells

M3 - Article

VL - 39

SP - 259

EP - 264

IS - 5

ER -