Prognostic value of sperm fluorescence in situ hybridization analysis over PGD

M. Sánchez-Castro, A. R. Jiménez-Macedo, M. Sandalinas, J. Blanco

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20 Citations (Scopus)

Abstract

Background: Sperm aneuploidy screening is now widely used as a counseling tool before performing a PGD cycle in infertile patients. The goal of this study was to determine whether sperm aneuploidy has a direct influence on embryo chromosomal abnormalities. Methods: Two groups were set up: (i) study group consisting of 13 oocyte-donation PGD cycles from males with normal karyotype and a previous altered sperm fluorescence in situ hybridization (FISH) result and (ii) control group including nine cycles of PGD for X-linked disease with no fertility problems and maternal age <36 years. Sperm samples and Day 3 embryos were evaluated using FISH for chromosomes X, Y, 13, 18 and 21. Results: A total of 179 embryos were analyzed: 91 embryos for the control group versus 88 for the study group. The study group presented more abnormal embryos than the control group (51.14 versus 35.16; P = 0.0353). Patients from the study group were then classified according to sperm count. Oligozoospermic patients showed a much higher proportion of abnormal embryos compared with the control group (64.87 versus 35.16; P = 0.0030). Conclusions: Sperm aneuploidy and diploidy screening seems to be an effective prognostic tool that would be useful in the reproductive genetic counseling of infertile couples, especially in oligozoospermic patients.
Original languageEnglish
Pages (from-to)1516-1521
JournalHuman Reproduction
Volume24
Issue number6
DOIs
Publication statusPublished - 1 Jan 2009

Keywords

  • Genetic counseling
  • Infertility
  • Oligozoospermia
  • PGD
  • Sperm fluorescence in situ hybridization

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