Production of thermally induced recombinant proteins relative to cell biomass is influenced by cell density in Escherichia coli batch cultures

José Luis Corchero, Pau Vila, Rafael Cubarsi, Antonio Villaverde

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4 Citations (Scopus)

Abstract

Fed-batch cultures of recombinant Escherichia coli were studied regarding their ability to permit CI857ts-controlled gene expression at different growth phases. Cells, growing at 50% pO2 and 28°C in a bioreactor, were transferred to a shaker flask at 42°C and the product yield was analysed after a short incubation time. Within the batch period, the synthesis of recombinant β-galactosidase relative to the increase of biomass during the induction period was enhanced in an biomass-dependent fashion. Whereas in young cells biomass production dominates over recombinant protein production, the available energetic resources of aged cells seem to be employed more efficiently for CI857-controlled biosynthesis of recombinant proteins than of cell material. A model to describe this 'altruistic' behaviour of old cultures is here proposed. © 1994 Chapman & Hall.
Original languageEnglish
Pages (from-to)777-782
JournalBiotechnology Letters
Volume16
Issue number8
DOIs
Publication statusPublished - 1 Aug 1994

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