Production of lipases by solid state fermentation using vegetable oil-refining wastes

Angélica Santis-Navarro, Teresa Gea, Raquel Barrena, Antoni Sánchez

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    Lipases were produced by a microbial consortium derived from a mixture of wastewater sludges in a medium containing solid industrial wastes rich in fats, under thermophilic conditions (temperature higher than 45 °C for 20 days) in 4.5-L reactors. The lipases were extracted from the solid medium using 100. mM Tris-HCl, pH 8.0 and a cationic surfactant agent (cetyltrimethylammonium chloride). Different doses of surfactant and buffer were tested according to a full factorial experimental design. The extracted lipases were most active at 61-65 °C and at pH 7.7-9. For the solid samples, the lipolytic activity reached up to 120,000. UA/g of dry matter. These values are considerably higher than those previously reported in literature for solid-state fermentation and highlight the possibility to work with the solid wastes as effective biocatalysts. © 2011 Elsevier Ltd.
    Original languageEnglish
    Pages (from-to)10080-10084
    JournalAgricultural Wastes
    Publication statusPublished - 1 Nov 2011


    • Lipase
    • Organic wastes
    • Sewage sludge
    • Solid-state fermentation
    • Thermostability


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