Production of HIV-1-based virus-like particles for vaccination: achievements and limits

Laura Cervera, Francesc Gòdia, Ferran Tarrés-Freixas, Carmen Aguilar-Gurrieri, Jorge Carrillo, Julià Blanco, Sònia Gutiérrez-Granados

Research output: Contribution to journalArticleResearchpeer-review

29 Citations (Scopus)

Abstract

© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. Over the past years, much knowledge has been gained about the HIV-1 virus structure and infection cycle. This knowledge has been used to conceive different types of potential vaccines and vaccination strategies. This review focuses on the characteristics of the virus and the vaccines that have been developed, particularly on those using virus-like particles, as well as on the developments for their production and purification. The production of HIV-1 VLPs has been investigated in different platforms such as, yeast, plants, insect and mammalian cells. Their purification follows the same rational as for viral vectors: clarification, nuclease treatment, concentration/capture, polishing, formulation and viral clearance. Analytical techniques to characterise the obtained productions will be of paramount relevance for their final application, considering that the raw production obtained in bioreactors comprises not only the VLPs of interest but also many other extracellular vesicles. Finally, it should also be considered that VLPs are prone to carry host cell proteins and DNA.
Original languageEnglish
Pages (from-to)7367-7384
Number of pages18
JournalApplied Microbiology and Biotechnology
Volume103
Issue number18
DOIs
Publication statusPublished - 1 Sept 2019

Keywords

  • HIV-1 VLPs
  • Production platforms
  • Purification strategies
  • Vaccines
  • Virus biology

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