Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations

Olga Durany; Philip Bassett; Amanda M.E. Weiss; Rocky M. Cranenburgh; Pau Ferrer; Josep López-Santín; Carles De Mas; Julian A.J. Hanak

doi:10.1002/bit.20527

Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations

Olga Durany, Philip Bassett, Amanda M.E. Weiss, Rocky M. Cranenburgh, Pau Ferrer, Josep López-Santín, Carles De Mas, Julian A.J. Hanak

Research output: Contribution to journal › Article › Research › peer-review

6 Citations (Scopus)

Abstract

We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems. © 2005 Wiley Periodicals, Inc.

Original language	English
Pages (from-to)	460-467
Journal	Biotechnology and Bioengineering
Volume	91
DOIs	https://doi.org/10.1002/bit.20527
Publication status	Published - 20 Aug 2005

Keywords

Escherichia coli
Fed-batch cultivation
Fuculose-1-phosphate aldolase
Heterologous protein production
Operator-repressor titration

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10.1002/bit.20527

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title = "Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations",

abstract = "We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems. {\textcopyright} 2005 Wiley Periodicals, Inc.",

keywords = "Escherichia coli, Fed-batch cultivation, Fuculose-1-phosphate aldolase, Heterologous protein production, Operator-repressor titration",

author = "Olga Durany and Philip Bassett and Weiss, {Amanda M.E.} and Cranenburgh, {Rocky M.} and Pau Ferrer and Josep L{\'o}pez-Sant{\'i}n and {De Mas}, Carles and Hanak, {Julian A.J.}",

year = "2005",

month = aug,

day = "20",

doi = "10.1002/bit.20527",

language = "English",

volume = "91",

pages = "460--467",

journal = "Biotechnology and Bioengineering",

issn = "0006-3592",

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TY - JOUR

T1 - Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations

AU - Durany, Olga

AU - Bassett, Philip

AU - Weiss, Amanda M.E.

AU - Cranenburgh, Rocky M.

AU - Ferrer, Pau

AU - López-Santín, Josep

AU - De Mas, Carles

AU - Hanak, Julian A.J.

PY - 2005/8/20

Y1 - 2005/8/20

N2 - We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems. © 2005 Wiley Periodicals, Inc.

AB - We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems. © 2005 Wiley Periodicals, Inc.

KW - Escherichia coli

KW - Fed-batch cultivation

KW - Fuculose-1-phosphate aldolase

KW - Heterologous protein production

KW - Operator-repressor titration

U2 - 10.1002/bit.20527

DO - 10.1002/bit.20527

M3 - Article

SN - 0006-3592

VL - 91

SP - 460

EP - 467

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

ER -

Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations

Abstract

Keywords

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