Production of fuculose-1-phosphate aldolase using operator-repressor titration for plasmid maintenance in high cell density Escherichia coli fermentations

Olga Durany, Philip Bassett, Amanda M.E. Weiss, Rocky M. Cranenburgh, Pau Ferrer, Josep López-Santín, Carles De Mas, Julian A.J. Hanak

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    6 Citations (Scopus)

    Abstract

    We report a novel application for the operator-repressor titration (ORT) plasmid maintenance system. The ability of ORT to maintain a plasmid during production of DNA has been demonstrated previously. In this study, we have used the ORT system to maintain a plasmid during high cell density cultivation and expression of a recombinant protein. No evidence of plasmid loss was seen during protein expression at high cell densities. In addition, the quantity of protein produced using this system was similar to traditional plasmid maintenance systems. © 2005 Wiley Periodicals, Inc.
    Original languageEnglish
    Pages (from-to)460-467
    JournalBiotechnology and Bioengineering
    Volume91
    DOIs
    Publication statusPublished - 20 Aug 2005

    Keywords

    • Escherichia coli
    • Fed-batch cultivation
    • Fuculose-1-phosphate aldolase
    • Heterologous protein production
    • Operator-repressor titration

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