Production of chimeric adenovirus

Marta Miralles; Marc Garcia; Marcos Tejero; Assumpció Bosch; Miguel Chillón

doi:10.1007/978-1-62703-679-5_16

Production of chimeric adenovirus

Marta Miralles, Marc Garcia, Marcos Tejero, Assumpció Bosch, Miguel Chillón

Department of Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The use of chimeric pseudotyped vectors is a common way to modify the adenoviral tropism by replacing the fiber protein. In this chapter the procedure to generate a chimeric adenovirus pre-stock from a plasmid containing the adenoviral genome is described. Also, the chimeric adenovirus replicative cycle to increase the yield in further productions is determined. Finally, two different protocols, in culture plates and in suspension cultures, to produce the virus at large scale are also detailed. © 2014 Springer Science+Business Media, LLC.

Original language	English
Pages (from-to)	231-243
Journal	Methods in Molecular Biology
Volume	1089
DOIs	https://doi.org/10.1007/978-1-62703-679-5_16
Publication status	Published - 1 Jan 2014

Keywords

Adenoviral vector
Adenovirus production
Chimeric vectors

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AB - The use of chimeric pseudotyped vectors is a common way to modify the adenoviral tropism by replacing the fiber protein. In this chapter the procedure to generate a chimeric adenovirus pre-stock from a plasmid containing the adenoviral genome is described. Also, the chimeric adenovirus replicative cycle to increase the yield in further productions is determined. Finally, two different protocols, in culture plates and in suspension cultures, to produce the virus at large scale are also detailed. © 2014 Springer Science+Business Media, LLC.

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