TY - JOUR
T1 - Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies
AU - Surribas, Anna
AU - Stahn, Rainer
AU - Montesinos, José Luis
AU - Enfors, Sven Olof
AU - Valero, Francisco
AU - Jahic, Mehmedalija
PY - 2007/6/30
Y1 - 2007/6/30
N2 - Different cultivation strategies have been compared for the production of Rhizopus oryzae lipase (ROL) from Pichia pastoris. Several drawbacks have been found using a methanol non-limited fed-batch. On the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. A temperature limited fed-batch has been proposed to solve both problems. However, in our case study a methanol non-limited fed-batch results in better productivities. Finally, a lower salt medium were used to overcome cell death problems and a temperature limited fed-batch was applied thereafter to solve oxygen transfer limitations. This combined strategy has resulted in lower productivities when compared to a methanol non-limited fed-batch. However the culture could be longer prolonged and a 1.3-fold purer final product was obtained mainly due to cell death reduction. © 2007 Elsevier B.V. All rights reserved.
AB - Different cultivation strategies have been compared for the production of Rhizopus oryzae lipase (ROL) from Pichia pastoris. Several drawbacks have been found using a methanol non-limited fed-batch. On the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. A temperature limited fed-batch has been proposed to solve both problems. However, in our case study a methanol non-limited fed-batch results in better productivities. Finally, a lower salt medium were used to overcome cell death problems and a temperature limited fed-batch was applied thereafter to solve oxygen transfer limitations. This combined strategy has resulted in lower productivities when compared to a methanol non-limited fed-batch. However the culture could be longer prolonged and a 1.3-fold purer final product was obtained mainly due to cell death reduction. © 2007 Elsevier B.V. All rights reserved.
KW - Cell death
KW - Operational fed-batch strategies
KW - Pichia pastoris
KW - Rhizopus oryzae lipase
U2 - https://doi.org/10.1016/j.jbiotec.2007.04.009
DO - https://doi.org/10.1016/j.jbiotec.2007.04.009
M3 - Article
VL - 130
SP - 291
EP - 299
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
ER -