Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies

Anna Surribas, Rainer Stahn, José Luis Montesinos, Sven Olof Enfors, Francisco Valero, Mehmedalija Jahic

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    37 Citations (Scopus)


    Different cultivation strategies have been compared for the production of Rhizopus oryzae lipase (ROL) from Pichia pastoris. Several drawbacks have been found using a methanol non-limited fed-batch. On the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. A temperature limited fed-batch has been proposed to solve both problems. However, in our case study a methanol non-limited fed-batch results in better productivities. Finally, a lower salt medium were used to overcome cell death problems and a temperature limited fed-batch was applied thereafter to solve oxygen transfer limitations. This combined strategy has resulted in lower productivities when compared to a methanol non-limited fed-batch. However the culture could be longer prolonged and a 1.3-fold purer final product was obtained mainly due to cell death reduction. © 2007 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)291-299
    JournalJournal of Biotechnology
    Publication statusPublished - 30 Jun 2007


    • Cell death
    • Operational fed-batch strategies
    • Pichia pastoris
    • Rhizopus oryzae lipase


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