Production of 5′-ribonucleotides by enzymatic hydrolysis of RNA

M. D. Benaiges; J. López-Santin; C. Solà

doi:10.1016/0141-0229(90)90078-5

Production of 5′-ribonucleotides by enzymatic hydrolysis of RNA

M. D. Benaiges, J. López-Santin, C. Solà

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

16 Citations (Scopus)

Abstract

Study of optimal operational conditions for RNA enzymatic hydrolysis to obtain 5′-ribonucleotides has been carried out. RNA from brewer's yeasts, obtained by ammonium extraction, was hydrolysed by a partially purified 5′-phosphodiesterase from barley rootlets. Temperature of 60°C and pH7 have been determined as the best operational conditions. Low RNA initial concentration (~0.1%) and reaction time (~1 h) have been identified as necessary to obtain a good yield of 5′-ribonucleotides. © 1990.

Original language	English
Pages (from-to)	86-89
Journal	Enzyme and Microbial Technology
Volume	12
Issue number	2
DOIs	https://doi.org/10.1016/0141-0229(90)90078-5
Publication status	Published - 1 Jan 1990

Keywords

5′-phosphodiesterase
5′-ribonucleotides
Enzymatic hydrolysis
RNA

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abstract = "Study of optimal operational conditions for RNA enzymatic hydrolysis to obtain 5′-ribonucleotides has been carried out. RNA from brewer's yeasts, obtained by ammonium extraction, was hydrolysed by a partially purified 5′-phosphodiesterase from barley rootlets. Temperature of 60°C and pH7 have been determined as the best operational conditions. Low RNA initial concentration (~0.1%) and reaction time (~1 h) have been identified as necessary to obtain a good yield of 5′-ribonucleotides. {\textcopyright} 1990.",

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AU - López-Santin, J.

AU - Solà, C.

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N2 - Study of optimal operational conditions for RNA enzymatic hydrolysis to obtain 5′-ribonucleotides has been carried out. RNA from brewer's yeasts, obtained by ammonium extraction, was hydrolysed by a partially purified 5′-phosphodiesterase from barley rootlets. Temperature of 60°C and pH7 have been determined as the best operational conditions. Low RNA initial concentration (~0.1%) and reaction time (~1 h) have been identified as necessary to obtain a good yield of 5′-ribonucleotides. © 1990.

AB - Study of optimal operational conditions for RNA enzymatic hydrolysis to obtain 5′-ribonucleotides has been carried out. RNA from brewer's yeasts, obtained by ammonium extraction, was hydrolysed by a partially purified 5′-phosphodiesterase from barley rootlets. Temperature of 60°C and pH7 have been determined as the best operational conditions. Low RNA initial concentration (~0.1%) and reaction time (~1 h) have been identified as necessary to obtain a good yield of 5′-ribonucleotides. © 1990.

KW - 5′-phosphodiesterase

KW - 5′-ribonucleotides

KW - Enzymatic hydrolysis

KW - RNA

U2 - 10.1016/0141-0229(90)90078-5

DO - 10.1016/0141-0229(90)90078-5

M3 - Article

VL - 12

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