Production of 5′-ribonucleotides by enzymatic hydrolysis of RNA

M. D. Benaiges, J. López-Santin, C. Solà

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

Study of optimal operational conditions for RNA enzymatic hydrolysis to obtain 5′-ribonucleotides has been carried out. RNA from brewer's yeasts, obtained by ammonium extraction, was hydrolysed by a partially purified 5′-phosphodiesterase from barley rootlets. Temperature of 60°C and pH7 have been determined as the best operational conditions. Low RNA initial concentration (~0.1%) and reaction time (~1 h) have been identified as necessary to obtain a good yield of 5′-ribonucleotides. © 1990.
Original languageEnglish
Pages (from-to)86-89
JournalEnzyme and Microbial Technology
Volume12
Issue number2
DOIs
Publication statusPublished - 1 Jan 1990

Keywords

  • 5′-phosphodiesterase
  • 5′-ribonucleotides
  • Enzymatic hydrolysis
  • RNA

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