A rhamnose-containing microbial polysaccharide has been produced by Klebsiella sp. I-714. strain. The polysaccharide has been used as a source for the obtention of L-rhamnose. A biotechnological process has been developed to purify the hydrolyzed polysaccharide (EPSH), which contains rhamnose, galactose and glucuronic acid. Microbial removal of galactose is followed by a continuous chromatographie separation of glucuronic acid to render pure rhamnose. The technical feasibility of the process has been studied, with special emphasis on microbial inhibitor's removal. © 1995 Springer-Verlag.