Processing and immobilization of enzyme Ribonuclease A through laser irradiation

C. Popescu, J. Roqueta, A. Pérez Del Pino, M. Moussaoui, M. V. Nogués, E. György

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

We report the processing and immobilization of enzyme Ribonuclease A (RNase A) onto SiO2 glass collectors using the matrix assisted pulsed laser evaporation (MAPLE) technique. The experiments were performed inside a stainless steel irradiation chamber. A pulsed UV KrF* (λ = 248 nm, τFWHM ≈ 25 ns, ν = 10 Hz) excimer laser source was used for the irradiations. The laser fluence was varied in the range 0.4-0.7 J/cm 2. The morphology of the obtained films was investigated by atomic force microscopy (AFM) and their structure and composition by Fourier transform infrared spectroscopy (FTIR). The FTIR spectra of the films obtained from composite MAPLE targets consisting of 1% (w/v) RNase A in Hepes-KOH 10 mM pH 7.5 buffer exhibit the same bands as the spectrum of the initial, nonirradiated material. The enzymatic activity of the obtained structures was analyzed using synthetic substrate polycytidylic acid (poly(C)). The poly (C) cleavage by the immobilized enzyme and the products of formation were analyzed by means of reverse phase high performance liquid chromatography (HPLC). © 2011 Materials Research Society.
Original languageEnglish
Pages (from-to)815-821
JournalJournal of Materials Research
Volume26
DOIs
Publication statusPublished - 28 Mar 2011

Keywords

  • Biomaterial
  • Laser
  • Radiation Effects

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