Primary alloproliferative TH1 response induced by immature plasmacytoid dendritic cells in collaboration with myeloid DCs

M. Naranjo-Gómez, M. A. Fernández, M. Bofill, R. Singh, C. V. Navarrete, R. Pujol-Borrell, F. E. Borràs

    Research output: Contribution to journalArticleResearchpeer-review

    10 Citations (Scopus)

    Abstract

    The role played by dendritic cell (DC) subsets in the immune response to alloantigens is not well defined. In vitro experiments have extensively shown that freshly isolated myeloid (M)DCs induce a strong T lymphocyte proliferation whereas plasmacytoid (P)DCs do not, unless activated by CD40 ligation. The aim of these studies was to explore whether the interplay among PDCs, MDCs and T cells modulates alloresponse. Freshly isolated MDCs and PDCs were merged in different proportions and used as antigen presenting cells (APCs) in mixed lymphocyte cultures (MLC). As described, isolated PDCs only induced a mild alloresponse, while MDCs were potent inducers of alloproliferation. Unexpectedly, when PDCs were merged with even low numbers of MDCs (down to 100 cells) and used as APCs, a potent Th1 cell proliferation was detected. Survival and maturation of PDCs was increased in these MLC conditions, which could partially explain the magnitude of the T-cell response. Interestingly, the proportion of IFNγ-producing cells generated in such cultures was higher compared to MDC-stimulated cultures. These data suggest that the interaction between both DC subsets is determinant to generate a potent Th1 response, at least in an allogeneic situation, and may be relevant to the outcome of allogeneic stem cell transplantation. Copyright © Blackwell Munksgaard 2005.
    Original languageEnglish
    Pages (from-to)2838-2848
    JournalAmerican Journal of Transplantation
    Volume5
    DOIs
    Publication statusPublished - 1 Dec 2005

    Keywords

    • Alloresponse
    • Dendritic cells
    • Graft versus host disease
    • Plasmacytoid DCs
    • Stem cell transplantation

    Fingerprint Dive into the research topics of 'Primary alloproliferative TH1 response induced by immature plasmacytoid dendritic cells in collaboration with myeloid DCs'. Together they form a unique fingerprint.

    Cite this